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Accelerated enzyme evolution for targeted C–H oxidation in cyclic amines

Chemical reagents readily oxidize alkyl amines at sites adjacent to nitrogen. Achieving selective oxidation at specified non-adjacent sites is much more challenging. A computation-guided process is introduced that enables rapid optimization of enzymes for site-selective hydroxylation of a variety of cyclic amines.

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Fig. 1: Docking-guided mutagenesis.

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This is a summary of: Zhang, Y. et al. C–H bonds in cyclic amines by iterative docking-guided mutagenesis of P450BM3 (CYP102A1). Nat. Synth. https://doi.org/10.1038/s44160-022-00166-6 (2022).

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Accelerated enzyme evolution for targeted C–H oxidation in cyclic amines. Nat. Synth (2022). https://doi.org/10.1038/s44160-022-00170-w

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  • DOI: https://doi.org/10.1038/s44160-022-00170-w

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