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GENOME INFORMATICS

Bridge over troubled transcripts

Scallop2, a tool that enables accurate reference-guided transcriptome assembly, capitalizes on recent developments in short-read sequencing protocols for single-cell RNA sequencing by leveraging multi-end and paired-end information.

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Fig. 1: Scallop2 uses UMIs to infer additional information from splice graphs.

References

  1. Conesa, A. et al. Genome Biol. 17, 13 (2016).

    Article  Google Scholar 

  2. Luecken, M. D. & Theis, F. J. Mol. Syst. Biol. 15, e8746 (2019).

    Article  Google Scholar 

  3. Aldridge, S. & Teichmann, S. A. Nat. Commun. 11, 4307 (2020).

    Article  Google Scholar 

  4. de Klerk, E. & ‘t Hoen, P. A. C. Trends Genet. 31, 128–139 (2015).

    Article  Google Scholar 

  5. Lebrigand, K., Magnone, V., Barbry, P. & Waldmann, R. Nat. Commun. 11, 4025 (2020).

    Article  Google Scholar 

  6. Martin, J. et al. BMC Genomics 11, 663 (2010).

    Article  Google Scholar 

  7. Zhang, Q., Shi, Q. & Shao, M. Nat. Comput. Sci. https://doi.org/10.1038/s43588-022-00216-1 (2022).

    Article  Google Scholar 

  8. Hagemann-Jensen, M. et al. Nat. Biotechnol. 38, 708–714 (2020).

    Article  Google Scholar 

  9. Heber, S., Alekseyev, M., Sze, S.-H., Tang, H. & Pevzner, P. A. Bioinformatics 18 Suppl 1, S181–S188 (2002).

  10. Westoby, J., Artemov, P., Hemberg, M. & Ferguson-Smith, A. Genome Biol. 21, 74 (2020).

    Article  Google Scholar 

Download references

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Correspondence to Martin Hemberg.

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Parada, G.E., Hemberg, M. Bridge over troubled transcripts. Nat Comput Sci 2, 142–143 (2022). https://doi.org/10.1038/s43588-022-00219-y

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