Extended Data Fig. 4: APJ expresses oligodendrocyte in the brain. | Nature Aging

Extended Data Fig. 4: APJ expresses oligodendrocyte in the brain.

From: Age-dependent decline in remyelination capacity is mediated by apelin–APJ signaling

Extended Data Fig. 4

a, Representative images of brain sections prepared from 3-week-old wild-type mice with O4 (green) and Olig2 (magenta) labeling. n = 3 biologically independent animals. b, Representative images of brain sections prepared from 3-week-old wild-type mice with PDGFRα or APC (green) and APJ (magenta) labeling. n = 3 biologically independent animals. c, Representative images of brain sections prepared from 3-week-old wild-type mice with NeuN, lectin, or GFAP (magenta) labeling and APJ (green) labeling. n = 3 biologically independent animals. d, Representative images of brain sections prepared from 3-week-old wild-type mice labeled with MAG and PLP-specific antibodies. Quantitation of the MAG- and PLP-negative areas in the corpus callosum (n = 3), P < 0.001 (MAG), 0.049 (PLP). e, Representative images of brain sections prepared from 4-week-old cKO mice and control mice with O4 and APJ labeling. Quantitation of APJ expression in O4-labeled cells (n = 3), P = 0.004. Scale bars, 200 μm for d, 20 μm for others. Data are the mean ± s.e.m. and were assessed using two-tailed Welch’s t-test.

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