a, Representative images of spinal cords labeled with Black Gold. Mice were treated with Pyr-apelin-13 15 days after EAE induction, and spinal cords were collected 30 days after EAE induction. Quantitation of the Black Gold-negative area in the spinal cord (n = 4). Each data point represents biologically independent animals. b, Change in the EAE score of mice treated with Pyr-apelin13 over time (n = 8). P = 0.011, 0.02 (left to right). Scale bars, 200 μm. Data are the mean ± s.e.m. and were assessed using two-tailed Welch’s t-test (a) and Mann-Whitney’s U test (b).