Extended Data Fig. 6: APJ-mediated remyelination is independent of inflammation. | Nature Aging

Extended Data Fig. 6: APJ-mediated remyelination is independent of inflammation.

From: Age-dependent decline in remyelination capacity is mediated by apelin–APJ signaling

Extended Data Fig. 6

a, The number of splenocytes obtained from mice after EAE induction. Mice were treated with ML233 15 days after EAE induction. Samples were prepared 28 days after EAE induction (n = 3). Each data point represents biologically independent animals. P = 0.571. b, Relative BrdU incorporation into the cultured splenocyte. Mice were treated with ML233 15 days after EAE induction. Samples were prepared 28 days after EAE induction (n = 4 for control, 3 for ML233). Each data point represents biologically independent animals. P = 0.121. c, Representative images of spinal cord sections stained with hematoxylin and eosin. Mice were treated with ML233 15 days after EAE induction. Samples were prepared 28 days after EAE induction (n = 3). Each data point represents biologically independent animals. P = 0.393. d, Representative flow cytometric contour plots show IFN-γ and IL-17A expression in CD45+ CD4+ T cells in the spinal cord. Immune cells were gated according to side scatter (SSC) and forward scatter (FSC) level (red line), and CD45+ CD4+ double-positive T cells were further gated (red rectangle). Samples were prepared 28 days after EAE induction. Right panels show the percentage of the IFN-γ (upper panel) and IL-17A-positive cells (lower panel) in the spinal cord (n = 4 for control, 3 for ML233), P = 0.96 (IFN-γ), 0.952 (IL-17). e, Relative expression of indicated cytokine in the supernatant of splenocyte culture. Splenocyte were obtained from EAE mice 2 weeks after ML233 treatment (n = 4). Each data point represents biologically independent experiments. Scale bar, 200 μm. Data are the mean ± s.e.m. and were assessed using two-tailed Welch’s t-test.

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