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Best practices for characterizing RNA lipid nanoparticles

To improve early-stage research in the field of RNA lipid nanoparticles, there are several best practices to be considered for the collection, interpretation and reporting of characterization data.

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Fig. 1: Properties that impact LNP characterization.


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The author thanks the members of the O.F.K. lab and the University of Toronto’s Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund (CFREF-2020). The author also thanks the New Frontiers in Research – Exploration stream (NFRFE-2022-00691), the Canada Research Chairs Program (CRC-2020-00097), the Natural Sciences and Engineering Research Council of Canada (RGPIN-2021-03421), the Canadian Institutes of Health Research (PTT-184009), and the Canada Foundation for Innovation John R. Evans Leaders Fund/Ontario Research Fund (41323) for support.

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Correspondence to Omar F. Khan.

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Khan, O.F. Best practices for characterizing RNA lipid nanoparticles. Nat Rev Methods Primers 3, 65 (2023).

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