New nanomaterials are being developed for efficient biomolecule delivery to plants. However, detection and quantification of plant cell entry are challenging and currently rely on subjective methods that lack proper controls. The necessary considerations of performing nanoparticle-mediated delivery in plants and how to accurately quantify delivery efficiency are discussed.
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The author thanks the Resnick Sustainability Institute at Caltech and the Henry Luce Foundation for funding.
The author declares no competing interests.
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Demirer, G.S. Detecting and quantifying nanoparticle-mediated biomolecule delivery in plants. Nat Rev Methods Primers 3, 16 (2023). https://doi.org/10.1038/s43586-023-00213-2