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Spectral cytometry on highly autofluorescent samples

Engineered materials induce immune responses when implanted in vivo. The autofluorescence spectra of responding cells can be captured by spectral cytometry. We discuss the necessary considerations when performing spectral cytometry on highly autofluorescent samples to extract phenotypic information from autofluorescence spectra and perform accurate quantification of fluorescent labels.

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Fig. 1: Characterizing highly autofluorescent samples with spectral cytometry.


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This work was funded by the Intramural Research Program of the National Institute for Biomedical Imaging and Bioengineering, and the National Cancer Institute, CCR, CIL of the National Institutes of Health, Bethesda, MD, USA.

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Correspondence to Matthew T. Wolf or Kaitlyn Sadtler.

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The authors declare no competing interests.

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The contents of this publication are the sole responsibility of the authors and do not necessarily reflect the views, opinions, or policies of the National Institutes of Health (NIH) and the Department of Health and Human Services (HHS). Mention of trade names, commercial products, or organizations does not imply endorsement by the US Government.

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Lokwani, R., Chaudhari, R., Wolf, M.T. et al. Spectral cytometry on highly autofluorescent samples. Nat Rev Methods Primers 2, 71 (2022).

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