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Genuine replication and pseudoreplication

A large sample size, or N, increases the sensitivity of an experiment to detect differences between treatment groups. However, the biological entity that N refers to may not be obvious. Defining the wrong entity can inflate the sample size and increase both false-positive and false-negative results.

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Correspondence to Stanley E. Lazic.

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Lazic, S.E. Genuine replication and pseudoreplication. Nat Rev Methods Primers 2, 23 (2022). https://doi.org/10.1038/s43586-022-00114-w

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