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Recent developments in automated single-particle selection

Automated single-particle picking in electron cryo-microscopy data has seen important advances in the past couple of years and now enables computer-assisted particle selection even for challenging datasets. These advances have implications for streamlined and automated image processing, with potential benefits for improving the resolution of resulting structures.

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Fig. 1: Selected filaments by SPHIRE-crYOLO in tomography mode.


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Correspondence to Stefan Raunser.

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Wagner, T., Raunser, S. Recent developments in automated single-particle selection. Nat Rev Methods Primers 2, 16 (2022).

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