Spatially offset Raman spectroscopy (SORS) is a spectroscopic technique that allows for the non-invasive chemical characterization of diffusely scattering materials, ranging from opaque plastics to biological tissues. SORS has been explored for a range of applications, including disease diagnosis, the detection of explosives through unopened containers and the in-depth, non-destructive analysis of pharmaceutical products and objects of art. This Primer introduces the reader to the basic concepts underpinning SORS, details best practices for its implementation, highlights its use across multiple fields and provides insight into its limitations. The Primer concludes by discussing potential applications and envisaging future developments in the field.
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This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) grant EP/R020965/1.
The authors declare that they are bound by confidentiality agreements that prevent them from disclosing their competing interests in this work.
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ChemSpider database: http://www.chemspider.com/Spectra.aspx?st=R
Gaussian software package: https://gaussian.com/
KnowItAll Raman Spectral Database Collection: https://sciencesolutions.wiley.com/solutions/technique/raman/knowitall-raman-collection/
- Rayleigh scattering
The elastic scattering of electromagnetic radiation by particles smaller than the wavelength of the radiation.
The degree to which a molecular dipole changes in response to an external electric field.
- Raman scattering
The inelastic scattering of photons, where the frequency of the scattered photon is different from that of the incident photon.
- Photon shot noise
Fluctuations of the detected number of photons, caused by the inherent particle-like properties of photons.
- Lambert’s cosine law
A law describing the cosine dependence of light emission intensity with respect to the angle of incidence from the surface normal.
- Solid angle
A measure of the amount of the field of view that an object occupies from a particular point.
- Acceptance angle
The maximum incidence angle of an optical ray that is transmitted to the spectrograph, measured from the optical axis of the spectrograph.
- Cosmic rays
High-energy protons and atomic nuclei that move through space at nearly the speed of light.
- Surface plasmon resonance
A resonant oscillation of nanoparticle conduction electrons induced by incident light; its spectral properties are dependent on nanoparticle size, shape and metal type.
- Monte Carlo simulations
Numerical algorithms that rely on the random sampling of events.
- Imaging phantoms
Specially prepared samples that mimic the properties of real biological tissue for the purposes of optical imaging.
Identical chemicals of different crystalline forms.
Wave-like modulation of charge-coupled device (CCD) sensitivity across the sensor caused by light interference and associated with back-illuminated CCDs.
- Read-out noise
Noise induced by charge digitization circuitry, imprinted on the signal when it is read.
- Thermal noise
Noise induced by thermal fluctuations of charge carriers within a detection element.
- Instrument response function
In the context of spatially offset Raman spectroscopy, a combined spectrograph–detector spectral intensity profile in response to illumination by a spectrally uniform light source.
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Cite this article
Mosca, S., Conti, C., Stone, N. et al. Spatially offset Raman spectroscopy. Nat Rev Methods Primers 1, 21 (2021). https://doi.org/10.1038/s43586-021-00019-0
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