## Introduction

The 2017 Minamata Convention on Mercury (Hg), a legally binding international treaty, aims to protect human health and the environment from the impact of Hg pollution by curtailing anthropogenic Hg emissions. However, knowledge gaps in global Hg cycling challenge our ability to assess the effectiveness of the Convention in reducing human and wildlife Hg exposure1,2,3,4. For example, the fate of the large quantity of Hg stored in the permafrost regions, estimated at 600–1700 Gg in permafrost soil of 0–3 m depth5,6, is poorly constrained. In a warming climate, thawing of permafrost may release this legacy Hg into the atmosphere at a rate that could impact global ecosystems5,6,7,8,9, and possibly offset the emission reduction efforts for anthropogenic Hg. After the Hg release, vegetation succession and biomass growth in the cryosphere promote atmospheric elemental Hg (Hg0) uptake and act as an active sink10,11. However, little is known about the cycling of Hg in the alpine permafrost regions outside of the Arctic.

Located at mid-latitude (26–40oN), the Qinghai-Tibet Plateau (QTP) hosts the largest alpine permafrost clusters in the world, covering an area of ~1.2 × 106 km2 and representing ~40% of global alpine permafrost areas8,12,13. The region has experienced accelerated warming, wetting, and thawing over recent decades12,14. Earlier studies have estimated 21.7 Gg of Hg stored in the surface 3-m of permafrost soils in the QTP, which is equivalent to 8% of the permafrost Hg pool in the Northern Hemisphere8. Climate-induced landscape perturbations and changes in vegetation and hydrogeochemical processes in the QTP permafrost regions may influence Hg biogeochemical cycling6,7,9,15, and may lead to rapid release of legacy Hg to the atmosphere. Thus, understanding Hg cycling in this alpine permafrost region can help assess the ecological and human health risk caused by Hg in the QTP and downstream regions, as well as the collective impact of thawing permafrost to global Hg cycling in a changing climate.

Earlier studies suggest the importance of root uptake16,17,18 but recent studies confirm that foliage uptake of atmospheric Hg0 is actually the dominant uptake pathway of Hg in most aboveground vegetation10,11,19,20,21,22,23,24, with the exception of selected aquatic vegetation in wetlands25,26. This is because Hg is largely blocked in the root zone by cell wall membranes27,28, resulting in limited root uptake (<10%) and transfer to aboveground biomass25. At elevations of more than 4000 m above sea level, the severe climate (e.g., low air pressure and temperature, strong wind, low precipitation, intense solar ultraviolet (UV)) in the QTP alpine permafrost promotes enhanced root growth and vegetation interaction with thawing permafrost soil29,30. We hypothesize that the combination of geochemical and ecological processes driven by the extreme climatic conditions, such as strong UV irradiance, and high elevation in QTP, promote Hg accumulation pathways in vegetation that differ from those observed in low-land regions.

Characteristics of Hg stable isotopes, including mass-dependent fractionation (MDF; the change of stable isotope composition due to a process of interest depends on the atomic mass of the isotope; reported as δ202Hg) and mass-independent fractionation (MIF; the change of stable isotope composition due to the process of interest does not scale in proportion to the masses of individual isotopes; reported as Δ199Hg and Δ201Hg for odd-MIF and Δ200Hg for even-MIF), offer new insight in identifying Hg sources and understanding accumulation processes in the ecosystems. Hg from atmospheric deposition and geogenic sources displays distinctly unique Hg isotopic signatures. Biogeochemical processes of Hg in the environment also exhibit unique isotopic fractionations10,11,19,20,21,22,23,24. The primary cause of MIF of Hg is the photochemical reduction of Hg(II) species, and the magnitude of this MIF of Hg depends on the complexing ligand and reaction conditions. For example, during photoreduction of Hg(II) by low-molecular-weight organic compounds, S-containing ligands promote preferential enrichment of 199Hg and 201Hg in the product of Hg0, while ligands without sulfur promote enrichment of 199Hg and 201Hg in the reactant (Hg(II))31,32,33.

Here we report the isotopic distribution of Hg in seasonally frozen-soil and permafrost regions of the QTP to quantify the sources, accumulation, and post-depositional processes of Hg in vegetation and soils. The study was conducted at the permafrost and seasonally frozen ground sites of the Sanjiangyuan (Three-River Headwaters) National Nature Reserve and the Nu River Headwaters Region. The region covers more than 2000 m elevation difference over ~5.0 × 105 km2 area (Supplementary Fig. 1a–c). Known as the “Asian Water Tower”, it is the origin of major river systems of the world. The vegetation cover is mainly alpine meadow and steppe, whose distributions vary with local climatic conditions and biological adaptability. Twenty-one sites were studied in June 2020, including 13 in alpine meadows and 8 in alpine steppes. Four sites (S01, S11, S19, and S21) have seasonally frozen soil ground, and 17 sites have permafrost cover (Fig. 1a and Supplementary Fig. 1). We determined the Hg concentration and isotopic signatures of grass foliage, root, rhizospheric soils, surface soils (0–17 cm depth herein), deep soils (more than 25 cm depth herein), and bedrock; then assessed the Hg spatial distribution and accumulation sources in vegetation and soils by Hg isotopic mixing models.

## Results and discussion

### Spatial distribution of Hg signatures

Grass root has the highest observed Hg concentration while bedrock has the lowest (p < 0.05, by One-Way ANOVA test, Fig. 1b). The measured Hg concentrations in grass foliage were comparable to those found in foliage of steppes and meadows (p = 0.65 by Kruskal-Wallis test, Supplementary Fig. 2), suggesting grass type is not the main factor influencing variations in foliar Hg concentration. The Hg concentration and pool size in grass foliage did not differ significantly between the permafrost and seasonally frozen ground regions (all p > 0.05 by Kruskal-Wallis test), while the average Hg concentration and pool size in the surface soil of frozen ground regions were 35−45% higher than in soil of permafrost (Supplementary Fig. 3). The highest δ202Hg signatures were measured in the bedrock at −0.23 ± 0.50‰, while the lowest values of −1.49 ± 0.59‰ were measured in the grass foliage (p < 0.05 by Kruskal-Wallis test). Similar values in the range of −0.67‰ to −0.85‰ were found in the rhizospheric and surface soil (Fig. 1c). Except for the significantly lower Δ199Hg in the bedrock at −0.06 ± 0.04‰ (p < 0.05 by Kruskal-Wallis test), most Δ199Hg signatures were similar in vegetation biomass and surface soils (Fig. 1d). The Δ200Hg signatures were close to zero for all samples (Fig. 1e), and the 0−5 cm depth and rhizospheric soils had the lowest values (p < 0.05 by Kruskal-Wallis test).

Hg concentration in foliage did not vary significantly with latitude (Supplementary Fig. 4a). The foliar Hg pool, surface soil Hg concentration and surface soil Hg pool size all decreased with latitude. The Δ199Hg in surface soil increased with latitude (Supplementary Fig. 4b, c; Fig. 2a, b). The significant correlation between precipitation intensity and surface soil Hg concentrations (Fig. 2c) and pool size (Fig. 2d) suggests that rainfall influences the spatial distribution of atmospheric Hg deposition. Higher precipitation at lower latitude (Supplementary Fig. 1) may increase atmospheric Hg2+ deposition and enhance atmospheric Hg0 uptake by vegetation through greater biomass production. The effect of precipitation in promoting vegetation growth is clearly shown in Supplementary Fig. 5a. The soil organic matter (SOC) in the surface soil comes from decomposition of litter, with a higher soil SOC content acting to lower soil pH34. The significant correlation between soil Hg concentration and SOC (Supplementary Fig. 5b), anticorrelation between soil Hg concentration and pH (Supplementary Fig. 5c), and correlation between aboveground grass biomass and soil Hg pool sizes (Supplementary Fig. 5d) all indicate an important role of vegetation in promoting Hg accumulation in soils. In addition, the greater SOC produced by vegetative biomass likely also retains a larger amount of Hg in soil through processes such as complexation19,20. The Δ199Hg values of vegetative biomass (Fig. 2e) and surface soil (Fig. 2f) both increase with altitude. Given the reduced air pressure, lower temperature, higher UV irradiance and stronger winds at elevated altitude35, these correlations suggest that the change of climatic and environmental conditions along with altitude may influence the Hg accumulation in vegetation and soils.

### Mercury accumulation in vegetation

Since Hg uptake by vegetation causes little Hg-MIF19,36, the Hg-MIF is a useful tracing tool for identifying Hg sources in vegetation. The atmospheric Hg0 exhibits negative Δ199Hg and Δ200Hg signals, while atmospheric Hg2+ has the distinctly positive Δ199Hg and Δ200Hg signals. The reported Δ199Hg signatures in foliage of low-altitude ecosystems across the globe are −0.25 ± 0.18‰, similar to those of atmospheric Hg0 because of foliage uptake11,19,22,37,38. The observed Δ199Hg values in grass foliage at high altitude are significantly different from those recorded in low-attitude terrestrial ecosystems (Figs. 1d and 3a), with Δ199Hg in grass foliage showing a positive shift of 0.2‰ to 0.4‰ compared to the signals found in foliage of low-altitude regions. These data suggest that atmospheric Hg0 uptake is not an important pathway for foliage Hg uptake in the QTP region.

The atmospheric Hg2+ at the study sites has higher Δ199Hg values of 0.89 ± 0.18‰ (Supplementary Table 1) compared to the global average of 0.54 ± 0.30‰19,20, uncharacteristic of elevated terrains with little influence from direct anthropogenic emissions. This led us to examine the influence of deposited atmospheric Hg2+ to the cuticle of foliage, which might contribute to the positive Δ199Hg signatures in foliage. Δ200Hg shifts are caused by photo-oxidation in the troposphere and relatively insensitive to other Hg biogeochemical processes36,39, thus useful to identify Hg sources that are of atmospheric Hg2+ origin. The much smaller Δ200Hg values in the foliage than those found in precipitation suggest that the contribution from atmospheric Hg2+ deposition was negligible (Fig. 3b).

The strong correlation in odd-MIF signatures among foliage, root, and rhizospheric soil (Supplementary Table 2) suggests that root uptake of soil Hg might be an important pathway for Hg accumulation in foliage. Furthermore, Δ199Hg and δ202Hg of grass foliage show the following relationship with Hg concentration (Fig. 3c, d) except at Site 11, a wet meadow with positive Δ199Hg values likely influenced by the meltwater Hg inputs having positive Δ199Hg:22

$${\varDelta }^{199}{{{{{\rm{Hg}}}}}}=0.02\pm 0.01\times {{{{{\rm{Hg}}}}}}-0.20\pm 0.10\textperthousand ,{{{{{{\rm{R}}}}}}}^{2}=0.53$$
(1)
$${{{{{{\rm{\delta }}}}}}}^{202}{{{{{\rm{Hg}}}}}}=0.07\pm 0.01\times {{{{{\rm{Hg}}}}}}-2.45\pm 0.17\textperthousand ,{{{{{{\rm{R}}}}}}}^{2}=0.80$$
(2)

Extrapolating this relationship to a zero Hg concentration using Eq. (1) results in a Δ199Hg value of –0.20 ± 0.10‰, approaching Δ199Hg values commonly found for atmospheric Hg0 in remote regions of QTP (−0.20 ± 0.07‰, Supplementary Table 3)40. The intercept of Eq. (2) is consistent with the known MDF for foliage uptake of atmospheric Hg0, which has a global median MDF value of –2.80‰11,19,22,37,38. Lower Hg concentrations in roots and foliage were found at lower elevations at Site 06 and 01 (Supplementary Fig. 1d), with up to −0.08 to −0.05‰ of Δ199Hg, which were close to the signatures of atmospheric Hg0. The highest Δ199Hg of 0.37‰ was found at Site 05, a permafrost site at 5000 m above sea level (Supplementary Fig. 1d). Finally, the geochemical indicator of Ti (Titanium, more discussion in Supplementary Note 1), which is mainly found in root due to uptake from surrounding soils41,42,43, also supports the hypothesis of root Hg uptake due to a strong positive correlation of Hg and Ti in root and a weak negative correlation in soils (Supplementary Fig. 6).

Given the 0.3–0.6‰ shift of Δ199Hg and comparable Δ200Hg between endmembers of atmospheric Hg0 and surface soil Hg, the signal of Δ199Hg is a better tracer than Δ200Hg for determining Hg sources in grass vegetation. The Δ199Hg mixing model (Fig. 4a) shows that the contribution of Hg derived from soil surrounding roots is similar to that in foliage (67 ± 21% versus 70 ± 19%). Our findings suggest that ~70% Hg in foliage on the QTP is derived from soil-root-foliage transport, and ~30% Hg in vegetation from atmospheric Hg0 uptake.

The relatively large difference between ~0.00‰ of Δ200Hg in roots and 0.25 ± 0.19‰ in precipitation (Fig. 3b) suggests that wet Hg2+ deposition is not the main source of Hg2+ to soils. Available Hg for root uptake could be from desorption of soil Hg and therefore exhibits similar isotopic compositions as soil. We found that the contribution of root uptake of soil Hg increased with altitude (Fig. 4b), leading to more positive Δ199Hg signals in vegetative biomass at higher elevation as demonstrated by the increase in Δ199Hg in grass foliage and roots with altitude (Fig. 2e).

Atmospheric Hg0 uptake by foliage has been regarded as the dominant Hg source in vegetation in low-altitude ecosystems, but our data indicate that this is not the case in the QTP permafrost region10,11,19,20,21,22,23,24. The physiological mechanism underlying enhanced root uptake of soil Hg in elevated permafrost regions needs further study. A plausible explanation may be that the low temperature and air pressure, strong UV irradiance and dry air at high altitude impose stress and force vegetation to capture water and nutrients from thawing permafrost soil through root uptake for survival29,30, thus facilitating a greater soil Hg uptake by root. This can be partly supported by the vegetation morphologic and metabolic changes to adapt to the harsh climatic and environmental conditions in elevated regions. For example, the harsh climate suppresses foliage growth, but promotes lateral root growth and enhances the production of root exudate44,45,46,47. These vegetation morphologic and metabolic changes are beneficial for soil Hg uptake by root. The relatively stronger UV irradiance in the alpine permafrost region can distinctly reduce respiration and photosynthesis, which limits atmospheric Hg0 uptake by foliage44,45,46,47, in addition to enhancing Hg photo-reduction and volatilization from plant surfaces48. Additionally, since foliage uptake of air Hg0 progresses as a first-order process depending on the air Hg0 concentration19,49, a lower flux of foliage Hg0 uptake is expected given the lower mass concentration of Hg0 in the ambient air of QTP. Finally, different grass species may respond to the climatic and environmental conditions in the QTP at various degrees, and therefore the grass species may also influence the atmospheric Hg0 uptake. With the sampling methodology in this study, the impact caused by grass species could not be discerned. It is therefore possible that the difference in grass species could have introduced noises to the isotope signatures that did not follow the overall trend with elevations.

### Hg accumulation in surface soil

The similarity in Hg MIF characteristics in soil among 0–5 cm, 5–12 cm, and 12–17 cm depth suggest similar Hg biogeochemical processes in the uppermost surface soil (Fig. 1d, e). Three source endmembers have been identified for soil Hg, including atmospheric Hg0 uptake, atmospheric Hg2+ deposition and geogenic sources (i.e., weathering processes induced rock Hg release into soils)10,19,22,23,50,51. The strong UV radiation in the QTP may enhance photo-reduction in surface soil to induce a shift of odd-MIF, but without an even-MIF. Therefore, the Δ200Hg signatures in soil is a superior tracer to identify the Hg sources. Ti is selected as a tracing element for geogenic sources since it is conserved in chemical weathering processes52. Using Hg/Ti ratios and Δ200Hg signatures, the source contribution of Hg in the uppermost soil profiles includes 40−55% from atmospheric Hg0 deposition, 23−25% from precipitation Hg2+ and 23−38% from geogenic sources (Fig. 4c). The contribution of atmospheric Hg0 deposition decreases with soil depth with increasing contribution of geogenic sources (Fig. 4c).

The Δ199Hg in soil Hg decreases with increasing soil Hg concentration (Fig. 3c), which we attribute to a greater contribution from foliage uptake of atmospheric Hg0 (Fig. 4d) and smaller geogenic source contribution (Fig. 4e) at elevated sites. Latitude anticorrelates with atmospheric Hg0 contribution (Fig. 4f), confirming that higher vegetation biomass in lower latitude can increase the atmospheric Hg0 uptake. The estimated fraction of atmospheric Hg2+ input justifies the 0.08−0.20‰ value of Δ199Hg found in soil. However, this cannot explain the odd-MIF of soil Hg increasing with elevation (Fig. 2f) since no gradient trends are observed for the estimated fraction of atmospheric Hg2+ input. This is likely caused by post-depositional redox processes50.

The slope of Δ199Hg/Δ201Hg has been used as a diagnostic index to reflect post-depositional redox processes in the environment since the Hg photolitically and organic-matter mediated reactions yield unique slopes of Δ199Hg/Δ201Hg. Photochemical reduction of Hg2+ complexed to organic ligands induces positive MIF in the residual Hg2+ with a slope of 0.9–1.4 depending on reaction conditions (e.g., pH, dissolved oxygen and the type of organic ligands)32,33. Organic-matter-mediated dark redox induces a slope of 1.6 with the enrichment of odd isotopes in the reactions53,54. Photo-reduction of MeHg is known to cause a positive MIF with a slope of Δ199Hg/Δ201Hg of 1.636,55.

The slope of 1.3 for soil Δ199Hg/Δ201Hg (Fig. 5a) compared to 1.0 for atmospheric Hg2+ and Hg0 depositions across the globe10,19,22,23,50,51, indicates that post-depositional redox processes induce a positive odd-MIF shift in surface soils. However, the <2% MeHg to total Hg ratio and the weak correlation to δ202Hg and Δ199Hg (Supplementary Fig. 7) suggest that the transformation between total Hg and MeHg does not support this pathway being responsible for the distinct odd-MIF in the QTP. Flux measurements of 2−25 ng m−2 h−1 over permafrost soil at Site 05 and Site 14 suggest that solar irradiance enhances soil Hg0 evasion15,56,57. Although the Hg isotopic signatures were not quantified in the soil–air Hg0 flux in the QTP, controlled experiments indicate that strong UV and enrichment of organic carbon in the uppermost soils could facilitate Hg(II) reduction in the presence of organic ligands and secondary photolysis, which may induce a positive odd-MIF shift and slope of 1.3 in this study32,33,54.

### Comparison to observations in Arctic tundra

The Arctic tundra is underlain by permafrost. Earlier studies have reported the Hg distribution and sources in the vegetation and soil, specifically in Alaska and Siberia5,6,10,11,58. The Arctic tundra regions experience similar low ambient temperatures (annually average < 0 °C) and precipitation (annually average < 400 mm) as in the QTP. In contrast, the QTP alpine permafrost regions have more severe climate, e.g., low air pressure, strong wind and intense solar UV, due to its elevation of over 4000 m above sea level. We compared our results to the reported data in the Arctic tundra regions to display the difference of Hg accumulation pathways between the alpine permafrost and Arctic permafrost regions.

The observed slope of Δ199Hg/Δ201Hg of vegetation and soil samples in Alaska tundra regions is ~1.0 (Fig. 5a), which is different to the value of 1.3 found in this study. This suggests that the post-depositional processes in the QTP under high UV radiation (Supplementary Fig. 8) and low air pressure lead to the unique Hg reduction that has not been observed elsewhere in the Arctic tundra31,32. The significantly lower Hg concentration (Fig. 5b, p < 0.001, Independent Samples T-test) found in the vegetation and surface soil samples can be attributed to the different vegetation species in the QTP (alpine grass species, e.g., Kobresia myosuroides) and in the Arctic tundra (tussock grass, ericaceous shrubs, moss and lichen). Without a root and cuticle layer system, moss and lichen cell walls are easily accessible for atmospheric Hg to be absorbed over the entire surface during the lifespan21,59, leading to elevated Hg concentrations. A much smaller aboveground vegetation Hg pool in the QTP permafrost (3.3 ± 1.6 µg m−2, Supplementary Fig. 2) compared to the value of 29 µg m−2 in Alaska tundra11 also explains the significantly lower soil Hg concentration in the permafrost regions of QTP.

Biomass and soil samples in the Arctic tundra regions exhibit distinctly negative Δ199Hg signatures (Fig. 5c), but air samples show similar MIF characteristics. This suggests foliage uptake of atmospheric Hg0 being the predominant source10,11. Combining vegetation and soil Hg sources of this study, we estimate that 30 ± 19% Hg in foliage is from foliage uptake of atmospheric Hg0, 31 ± 17% from root uptake of previously deposited Hg0, 16 ± 15% from root uptake of previously deposited Hg2+, and 23 ± 20% from root uptake of previously geogenic Hg (more details for this calculation can be found in Materials and Methods). This may be due to strong UV and low air pressure in the permafrost regions of QTP forcing morphologic changes of vascular vegetation to adapt the extreme environmental conditions (e.g., root-to-shoot biomass ratio, R/S), leading to a change of Hg accumulation pathway in vegetation45,46. The R/S ratios of grassy species in the QTP permafrost regions nearly double the values found in the Arctic tundra regions (Fig. 5d, p < 0.001, Independent Samples T-test). A higher R/S ratio promotes nutrient uptake from soils60,61,62, thus enhancing root Hg uptake and translocation to foliage.

We recognize that uncertainty exists due to spatio-temporal variability and limited datasets. However, the data are indicative of Hg accumulation processes in the Arctic regions. Both foliage and root uptake pathways may exist simultaneously, but might differ in strength from place to place.

## Conclusions and implications

Our study suggests that vegetation adapts to the extreme environmental conditions in the alpine permafrost regions of the QTP, resulting in enhanced root uptake of Hg from surrounding soil and subsequent Hg translocation from root to foliage. Our findings indicate a process that counters the previously reported Hg accumulation pathways in the vegetation of low-altitude ecosystems. While both pathways exist, the contribution from foliage and root uptake by vegetation can be shaped by the environmental conditions.

Recent studies have revealed that the thawing of permafrost has stimulated vegetation productivity in recent decades in the QTP regions12,14. Therefore, an increase in atmospheric Hg0 deposition through vegetative uptake into these permafrost regions can be expected20,63,64. Assuming that weakened atmospheric Hg0 uptake by vegetation occurs in the 3.5 million km2 of global alpine permafrost regions (20−30% of total permafrost regions in the Northern Hemisphere)13, we estimate 6−17 Mg Hg distributed in vegetation of global alpine permafrost regions13 using the mean vegetation Hg pool size in this study. Given the 1-year lifespan of grass foliage in permafrost, this represents 2−5 Mg yr−1 of atmospheric Hg0 sink caused by foliage uptake, ~70% lower than the estimate using models that assume foliage Hg is derived from atmospheric Hg0.

## Materials and methods

### Site descriptions

Our study regions are located in Sichuan, Qinghai and Tibet provinces (91.6°E−102.8°E, 30.0°N−35.9°N, with an area of about 5.0 × 105 km2), including the Sanjiangyuan (Three-River Headwaters) National Nature Reserve (the headwaters of the Huanghe (Yellow River), Changjiang (Yangtze River) and Lancanjiang-Mekong River, with an area of 3.0 × 105 km2) and the headwater region of the Nujiang-Salween River (Supplementary Fig. 1). The study sites range in elevation from 3100 m to 5000 m above the sea level. The vegetation cover of these regions is mainly alpine meadow and steppe, which are distributed in different elevations due to the local climatic conditions and biological adaptability. The dominant grass species are Kobresia myosuroides (Villars) Foiri, Leontopodium leontopodioides (Willd.) Beauv, and Stipa capillata L. in alpine steppe, and Kobresia humilis Sergievskay, Poa pratensis L. and Stipa capillata L. in alpine meadow. Specifically, Kobresia humilis and Leontopodium nanum are dominated in the elevated regions. The soil pH ranges from 5.6 to 9.8 depending on the site locations. The climate is mainly controlled by the Indian and East Asian monsoon in the summer and the Westerlies in the winter65. The annual average temperature ranges from −1 °C to 1 °C, and annual precipitation from 400 mm to 600 mm in the seasonally frozen regions, and 200 mm to 400 mm in the permafrost regions. Data of annually precipitation for 2016 in the TGR are obtained from National Tibetan Plateau/Third Pole Environment Data Center (https://data.tpdc.ac.cn/en/data/3fc66dcd-5db9-4af4-bfc6-55671875a400/).

### Sample collections and measurements

Twenty-one sites were studied in June 2020. The distance between each site ranges from 20 to 350 km. The sampling site is chosen based on the criteria that there is at least 150 m of flat or nearly flat land in the surrounding area and that there are no distinct human activities except for slight livestock grazing in some of the meadows. The sampling locations were identified by global positioning system. At each sampling site, we drew six 5 m × 5 m sampling subplots, and collected samples of the uppermost layers of soils at a depth interval of 0–5 cm, 5–12 cm, and 12–17 cm, deep subsoils (from > 25 cm up to 100 cm depth, depending on the nature of the sites) and bedrocks. We also harvested the aboveground biomass of grass (referred to as grass foliage herein) and grass root from a 50 cm × 50 cm quadrat within a 10-cm depth in each sampling plot, and collected the rhizospheric soil samples from the surface of the grass root for analysis. All samples were put into clean polyethylene bags and stored immediately in a 4 °C portable refrigerator. In total, 200 vegetation and 480 soil samples were collected.

We sampled the precipitation and snow samples at the Sanjiangyuan Alipine Steppe Ecosystem Research Station (SJY, 34°22′N,100°30′E) in 2021. The sampling methodologies and QA/QC for precipitation and snow are similar to the discription in our ealier studies20,21. Briefly, 5 L of water was collected by a precipitation collector. Approximately 5 mL of BrCl and 200 μL SnCl2 were successively added into the water samples to purge and trap the generated Hg0 into the 1 g chlorinate carbon trap. This approach can releases 95 ± 7% of Hg from water samples.

Mercury concentrations in vegetation and soil samples were measured on a DMA80 Hg analyzer, following the protocol described in our earlier studies21,66. Briefly, the National Standard Reference Materials of China GBW07405 (GSS-5, soil, Hg = 290 ± 40 ng g−1), GBW10020 (GSB-11, vegetation, Hg = 150 ± 25 ng g−1) and GBW10049 (GSB-27, vegetation, Hg = 12 ± 3 ng g−1)) were used for QA/QC and measured after every 9 samples with a recovery of 95–105%. Soil organic carbon (SOC) content was determined by the Walkley-Back method which involves oxidation of SOCby by Cr2O72- followed by the addition of FeSO4 to reduce the excess Cr2O72-67. The concentration of titanium (Ti) in vegetation, soil and rock samples was analyzed by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES), following U.S. EPA Method 6020B. Briefly, about 0.1 g soil sample were digested in a closed Teflon vessel by an acid mixture (HNO3:HF = 5:1, v/v) at 150 °C for 48 hours. The digested solution was transferred quantitatively with ultra-pure water to a 50 mL Teflon bottle and analyzed by ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometer). The SRM, blank and replicated samples were analyzed in every 10 treatments (7 samples + 1 SRM + 1 blank + 1 replicated sample). The average percentages of Ti recovered were 94.5 ± 5.0%.

All soil samples were weighed 0.4–0.5 g and extracted by a mixture of CuSO4-HNO3-CH2Cl2 (shaken for half an hour). The quality control for the analytical process of MeHg was carried out by using duplicates and certified reference materials (ERM-CC580 and Tort-3). The MeHg recovery for certified reference materials was 90–110% and the relative standard deviations of the duplicate samples were < 10%.

The procedures for Hg isotope measurement have been described previously21,50,59. Briefly, all samples were processed by a double-stage tube furnace and trapping solutions (anti aqua regia, HNO3:HCl = 2:1, v/v) for Hg preconcentration, and the solutions were diluted to 0.5 ng mL−1 prior to Hg isotope measurement on a MC-ICP-MS (Multicollector-Inductively Coupled Plasma Mass Spectrometer, Nu-Plasma II). The acid strength of the diluted solution was about 10%. Hg standard (NIST-3133) and and Hg isotope standard (NIST-8610) solutions were matched to the sample solution (0.5 ng mL−1 Hg, 10% acid strength with anti-aqua regia). The Hg-MDF is reported in δ notation using the unit of permil (‰) referenced to the neighboring NIST-3133 solution:

$${{{{{{\rm{\delta }}}}}}}^{202}{{{{{\rm{Hg}}}}}}({{\textperthousand }})=1000\times [({\,\!}^{202}{{{{{\rm{Hg}}}}}}{/}^{198}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{sample}}}}}}})/{(}{\,\!}^{202}{{{{{\rm{Hg}}}}}}{/}^{198}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{NIST}}}}}}-3133})-1]$$
(3)

MIF is reported as ΔxxxHg following the convention suggested by Blum and Bergquist:68

$${\varDelta }^{199}{{{{{\rm{Hg}}}}}}(\textperthousand )={{{{{{\rm{\delta }}}}}}}^{199}{{{{{\rm{Hg}}}}}}-0.2520\times {{{{{{\rm{\delta }}}}}}}^{202}{{{{{\rm{Hg}}}}}}$$
(4)
$${\varDelta }^{200}{{{{{\rm{Hg}}}}}}(\textperthousand )={{{{{{\rm{\delta }}}}}}}^{200}{{{{{\rm{Hg}}}}}}-0.5024\times {{{{{{\rm{\delta }}}}}}}^{202}{{{{{\rm{Hg}}}}}}$$
(5)
$${\varDelta }^{201}{{{{{\rm{Hg}}}}}}(\textperthousand )={{{{{{\rm{\delta }}}}}}}^{201}{{{{{\rm{Hg}}}}}}-0.7520\times {{{{{{\rm{\delta }}}}}}}^{202}{{{{{\rm{Hg}}}}}}$$
(6)

BCR-482 (vegetation SRM) and GSS-4 (soil SRM) were combusted in an oven-enrichment system after every 10 samples to assess if the non-unity recoveries resulting from the double-stage offline combustion-trapping technique induced discernible isotopic bias; their recoveries ranged from 94% to 104%. The Hg isotopic signatures of BCR-482 were measured as δ202Hg = −1.67 ± 0.12‰, Δ199Hg = −0.56 ± 0.08‰, Δ200Hg = −0.01 ± 0.04‰ and Δ201Hg = −0.58 ± 0.08‰ (Mean ± 2 SD, Standard deviation, n = 6), and of GSS-4 as δ202Hg = −1.72 ± 0.16‰, Δ199Hg = −0.34 ± 0.06‰, Δ201Hg = −0.34 ± 0.06‰ and Δ200Hg = −0.00 ± 0.04‰ (Mean ± 2 SD, n = 6). The NIST-8610 was also analyzed for every 10 samples during the Hg isotope measurements, with isotopic signatures of δ202Hg = −0.53 ± 0.08‰, Δ199Hg = −0.00 ± 0.08‰ and Δ201Hg = −0.03 ± 0.06‰ (Mean ± 2 SD, n = 12). All measured Hg isotopic signatures of these certified reference materials (CRM) were consistent with their respective recommended values68,69.

### Hg isotope mixing model

Δ199Hg signals were utilized for estimating the contribution of root uptake soil Hg and foliage uptake atmospheric Hg0 in vegetation as:

$${\varDelta }^{199}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{soil}}}}}}}\times {f}_{soil-veg}+{\varDelta }^{199}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{air}}}}}}-{{{{{\rm{Hg}}}}}}(0)}\times (1-{f}_{soil-veg})={\varDelta }^{199}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{veg}}}}}}}$$
(7)

where fsoil−veg is the soil Hg source contribution, and Δ199Hgair−Hg(0) is the signature of atmospheric Hg0, and Δ199Hgveg is the signature of the vegetation foliage or root. The term “1−fsoil−veg” in Eq. (7) represents the contribution from live foliage uptake atmospheric Hg0. Given Δ200Hg signatures are insensitive to Hg biogeochemical modification in the ecosystems, we estimated the Hg sources in soils as:

$${f}_{geo}={({{{{{\rm{Ti}}}}}}/{{{{{\rm{Hg}}}}}})}_{{{{{{\rm{soil}}}}}}}\times {({{{{{\rm{Hg}}}}}}/{{{{{\rm{Ti}}}}}})}_{{{{{{\rm{crust}}}}}}}$$
(8)
$${f}_{geo}+{f}_{soil-Hg(0)}+{f}_{soil-Hg(II)}=1$$
(9)
$${\varDelta }^{200}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{geo}}}}}}}\times {f}_{geo} +{\varDelta }^{200}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{air}}}}}}-{{{{{\rm{Hg}}}}}}(0)}\times {f}_{soil-Hg(0)}+{\varDelta }^{200}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{air}}}}}}-{{{{{\rm{Hg}}}}}}({{{{{\rm{II}}}}}})}\\ \times {f}_{soil-Hg(II)}={\varDelta }^{200}{{{{{{\rm{Hg}}}}}}}_{{{{{{\rm{soil}}}}}}}$$
(10)

where fgeo is the contribution fraction for geogenic sources (Δ200Hggeo), and fsoil−Hg(0) is the total contribution fraction for atmospheric Hg0 sources (Δ200Hgair−Hg(0)), and fsoil−Hg(II) is the total contribution fraction for atmospheric Hg2+ sources (Δ200Hgair–Hg(II)). In addition, we also calculated root uptake of previously deposited Hg0 (fleg−Hg0) in soils as:

$${{{{{{\rm{f}}}}}}}_{{{{{{\rm{leg}}}}}}-{{{{{\rm{Hg0}}}}}}}={f}_{soil-veg}\times {f}_{soil-Hg(0)}$$
(11)

We used the intercept of Eq. (1) in the main text (−0.20 ± 0.10‰) to represent the Δ199Hgair−Hg(0), which agrees with exceptional well with recently reported atmospheric Hg0 signatures (−0.20 ± 0.07‰) at Nyingchi, a remote site in the QTP (n = 31, Supplementary Table 3)40. The value is also consistent with an earlier reported global average of Δ199Hg for atmospheric Hg0 (−0.16 ± 0.11‰, n = 343)19. We note that the rhizospheric soil samples were collected from 10 cm depth root, which may not represent the entire soil matrix from where root uptake of Hg takes place. Hence, we used the average Δ199Hg value of 0–17 cm soils and rhizospheric soils to represent the Δ199Hgsoil at each site.

The Δ200Hg signatures are caused by photo-oxidation in the troposphere and relatively insensitive to other Hg biogeochemical processes36,39, thus can be used as a powerful tracer to identify Hg sources that are atmospheric in origin. Using the Hg/Ti ratios and Δ200Hg signatures, we are able to quantify the contributions from various sources to Hg in the uppermost soil profiles. The average of Δ200Hg in rainfall across the QTP is 0.19 ± 0.05‰ (Supplementary Table 1) and the atmospheric Hg0 in the global remote regions has an average value of −0.05 ± 0.05‰19,70. Both mean values are used in our modeling.

Since the Hg isotopic signature of the soil solution is unknown, uncertainty exists in estimating the contribution of atmospheric Hg2+ sources in the isotopic mixing models. There is also a knowledge gap in the measurement of gaseous oxidized Hg71,72,73, resulting in analytical uncertainty of the reported MIF signatures. The oxidation of Hg0 in the tropopause is suggested as the main cause for the Δ200Hg signature in precipitation39. The stratospheric intrusion in the QTP may lead to the enrichment of atmospheric Hg2+ concentrations at higher elevations74,75. We assumed constant Δ199Hg and Δ200Hg values in atmospheric Hg inputs without considering elevational differences. Our earlier studies have suggested the Hg isotope mixing models associated with Monte Carlo simulations could reduce the uncertainties of the model results20,21. These uncertainties are quantified by generating one million groups of MIF signatures randomly ranging from Mean-2SD to Mean + 2 SD to solve the Hg isotope mixing models. Given at the 95% confidence level, our models’ uncertainty ranges between 16% and 40% (2 SD, details in Supplementary Fig. 915).

### Statistical methods

Data were analyzed by using the statistical program R 4.10 and SPSS 26.0 with p < 0.05 as the level of statistical significance. We used the generalized linear model (GLM) to analyze the spatial response among values of Hg concentration, pool size, isotopic signatures, altitude and precipitation. GLM is a generalization of ordinary linear regression that allows for response variables that have error distribution models other than a normal distribution like the Gaussian distribution. In addition, we used Independent Samples T-test, One-Way ANOVA and post hoc Tukey HSD to conduct the significant difference analysis when data were normally distributed; otherwise, we used the Kruskal-Wallis test.

### Reporting summary

Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.