Fig. 7: Cytocompatibility assays of protein fibrils. | Communications Materials

Fig. 7: Cytocompatibility assays of protein fibrils.

From: Instantaneous fibrillation of egg white proteome with ionic liquid and macromolecular crowding

Fig. 7

Live-Dead assay of a control cells and b in presence of 25% of protein fibrils (scale bar: 50 μm). Green channel: live cells–Calcein–AM, Red channel: necrotic cells–PI. Fluorescent micrographs of the morphology of c control cells and d cells incubated with 25% of protein fibrils (scale bar: 20 µm). Green channel: F-actin–fluorescein phalloidin, Blue channel: nucleus–DAPI. Optical micrographs of e control cells with no fibrils and f with cells incubated with 25% of the EWP fibrils stock solution (scale bar: 50 µm). g Viability plot of L929 cells in presence of different concentrations of protein fibrils (5–25%). Data are presented as average ± standard deviation (NS = no significant difference compared to the control (no fibrils).

Back to article page