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High-throughput coating with biodegradable antimicrobial pullulan fibres extends shelf life and reduces weight loss in an avocado model


Food waste and food safety motivate the need for improved food packaging solutions. However, current films/coatings addressing these issues are often limited by inefficient release dynamics that require large quantities of active ingredients. Here we developed antimicrobial pullulan fibre (APF)-based packaging that is biodegradable and capable of wrapping food substrates, increasing their longevity and enhancing their safety. APFs were spun using a high-throughput system, termed focused rotary jet spinning, with water as the only solvent, allowing the incorporation of naturally derived antimicrobial agents. Using avocados as a representative example, we demonstrate that APF-coated samples had their shelf life extended by inhibited proliferation of natural microflora, and lost less weight than uncoated control samples. This work offers a promising technique to produce scalable, low-cost and environmentally friendly biodegradable antimicrobial packaging systems.

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Fig. 1: Direct coating of avocados with rinsible PFs.
Fig. 2: Morphology and chemical composition of PFs and APFs.
Fig. 3: Direct contact assay of antimicrobial and antifungal activity of APFs.
Fig. 4: Effect of APF coating (5.0 mg cm2) on avocado rotting.
Fig. 5: The effect of APF coating on avocado colour, firmness and pH during storage at 22°C.

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Data availability

The data that support the findings of this study are available from the corresponding authors upon reasonable request. Source data are provided with this paper.


  1. Lee, H. & Yoon, Y. Etiological agents implicated in foodborne illness world wide. Food Sci. Anim. Resour. 41, 1–7 (2021).

    Article  Google Scholar 

  2. Hoffmann, S. & Ahn, J.-W. Economic cost of major foodborne illnesses increased $2 billion from 2013 to 2018. Amber Waves (2 April 2021).

  3. Vilela, C. et al. A concise guide to active agents for active food packaging. Trends Food Sci. Technol. 80, 212–222 (2018).

    Article  CAS  Google Scholar 

  4. Sharma, R., Jafari, S. M. & Sharma, S. Antimicrobial bio-nanocomposites and their potential applications in food packaging. Food Control 112, 107086 (2020).

    Article  CAS  Google Scholar 

  5. Mellinas, C. et al. Active edible films: current state and future trends. J. Appl. Polym. Sci. 133, 42631 (2016).

    Article  Google Scholar 

  6. Marelli, B., Brenckle, M., Kaplan, D. L. & Omenetto, F. G. Silk fibroin as edible coating for perishable food preservation. Sci. Rep. 6, 1–11 (2016).

    Article  Google Scholar 

  7. Göksen, G., Fabra, M. J., Ekiz, H. I. & López-Rubio, A. Phytochemical-loaded electrospun nanofibers as novel active edible films: characterization and antibacterial efficiency in cheese slices. Food Control 112, 107133 (2020).

    Article  Google Scholar 

  8. Wen, P. et al. Fabrication of electrospun polylactic acid nanofilm incorporating cinnamon essential oil/β-cyclodextrin inclusion complex for antimicrobial packaging. Food Chem. 196, 996–1004 (2016).

    Article  CAS  Google Scholar 

  9. Jafarzadeh, S. et al. Biodegradable green packaging with antimicrobial functions based on the bioactive compounds from tropical plants and their by-products. Trends Food Sci. Technol. 100, 262–277 (2020).

    Article  CAS  Google Scholar 

  10. Bhushani, J. A. & Anandharamakrishnan, C. Electrospinning and electrospraying techniques: potential food based applications. Trends Food Sci. Technol. 38, 21–33 (2014).

    Article  Google Scholar 

  11. Aytac, Z. et al. Development of biodegradable and antimicrobial electrospun zein fibers for food packaging. ACS Sustain. Chem. Eng. 8, 15354–15365 (2020).

    Article  CAS  Google Scholar 

  12. Aytac, Z. et al. Enzyme- and relative humidity-responsive antimicrobial fibers for active food packaging. ACS Appl. Mater. Interfaces 13, 50298–50308 (2021).

    Article  CAS  Google Scholar 

  13. Chang, H. et al. Structure–function in helical cardiac musculature using additive textile manufacturing. Preprint at bioRxiv (2021).

  14. Agency Response Letter, GRAS Notice No. GRN 000099 (US Food and Drug Administration, 2002).

  15. Munhuweyi, K., Mpai, S. & Sivakumar, D. Extension of avocado fruit postharvest quality using non-chemical treatments. Agronomy 10, 212 (2020).

    Article  CAS  Google Scholar 

  16. Karim, M. R. et al. Preparation and characterization of electrospun pullulan/montmorillonite nanofiber mats in aqueous solution. Carbohydr. Polym. 78, 336–342 (2009).

    Article  CAS  Google Scholar 

  17. Niaz, T. et al. Polyelectrolyte multicomponent colloidosomes loaded with nisin Z for enhanced antimicrobial activity against foodborne resistant pathogens. Front. Microbiol. 8, 2700 (2018).

    Article  Google Scholar 

  18. Trindade, G. G. G. et al. Carvacrol/β-cyclodextrin inclusion complex inhibits cell proliferation and migration of prostate cancer cells. Food Chem. Toxicol. 125, 198–209 (2019).

    Article  CAS  Google Scholar 

  19. Durmus, D. CIELAB color space boundaries under theoretical spectra and 99 test color samples. Color Res. Appl. 45, 796–802 (2020).

    Article  Google Scholar 

  20. Abdollahzadeh, E., Nematollahi, A. & Hosseini, H. Composition of antimicrobial edible films and methods for assessing their antimicrobial activity: a review. Trends Food Sci. Technol. 110, 291–303 (2021).

    Article  CAS  Google Scholar 

  21. Poudel, D. et al. Novel electrospun pullulan fibers incorporating hydroxypropyl-β-cyclodextrin: morphology and relation with rheological properties. Polymers 12, 2558 (2020).

    Article  CAS  Google Scholar 

  22. Xiao, Q. & Lim, L.-T. Pullulan–alginate fibers produced using free surface electrospinning. Int. J. Biol. Macromol. 112, 809–817 (2018).

    Article  CAS  Google Scholar 

  23. Tomasula, P. M. et al. Electrospinning of casein/pullulan blends for food-grade applications. J. Dairy Sci. 99, 1837–1845 (2016).

    Article  CAS  Google Scholar 

  24. Li, R. et al. Electrospinning pullulan fibers from salt solutions. Polymers 9, 32 (2017).

    Article  Google Scholar 

  25. Farris, S., Unalan, I. U., Introzzi, L., Fuentes-Alventosa, J. M. & Cozzolino, C. A. Pullulan-based films and coatings for food packaging: present applications, emerging opportunities, and future challenges. J. Appl. Polym. Sci. 131, 40539 (2014).

    Article  Google Scholar 

  26. Willingham, S. L. et al. Effects of rootstock and nitrogen fertiliser on postharvest anthracnose development in Hass avocado. Australas. Plant Pathol. 35, 619–629 (2006).

    Article  CAS  Google Scholar 

  27. Hartill, W. F. T. & Everett, K. R. Inoculum sources and infection pathways of pathogens causing stem-end rots of ‘Hass’ avocado (Persea americana). NZ J. Crop Hortic. Sci. 30, 249–260 (2002).

    Article  Google Scholar 

  28. Angelo, K. et al. Multistate outbreak of Listeria monocytogenes infections linked to whole apples used in commercially produced, prepackaged caramel apples: United States, 2014–2015. Epidemiol. Infect. 145, 848–856 (2017).

    Article  CAS  Google Scholar 

  29. Le, K. H. et al. A novel antimicrobial ZnO nanoparticles-added polysaccharide edible coating for the preservation of postharvest avocado under ambient conditions. Prog. Org. Coat. 158, 106339 (2021).

    Article  CAS  Google Scholar 

  30. Topuz, F. & Uyar, T. Antioxidant, antibacterial and antifungal electrospun nanofibers for food packaging applications. Food Res. Int. 130, 108927 (2020).

    Article  CAS  Google Scholar 

  31. Feng, K. et al. Enhancement of the antimicrobial activity of cinnamon essential oil-loaded electrospun nanofilm by the incorporation of lysozyme. RSC Adv. 7, 1572–1580 (2017).

    Article  ADS  CAS  Google Scholar 

  32. Garcia, F. & Davidov‐Pardo, G. Recent advances in the use of edible coatings for preservation of avocados: a review. J. Food Sci. 86, 6–15 (2021).

    Article  CAS  Google Scholar 

  33. Shi, L., Le Visage, C. & Chew, S. Y. Long-term stabilization of polysaccharide electrospun fibres by in situ cross-linking. J. Biomater. Sci. Polym. Ed. 22, 1459–1472 (2011).

    Article  CAS  Google Scholar 

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We thank the Nanyang Technological University–Harvard T. H. Chan School of Public Health Initiative for Sustainable Nanotechnology for funding support (project number NTUHSPH 18003). This work was performed in part at the Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the National Science Foundation under NSF award number 1541959 and at the Harvard MRSEC (grant numbers DMR-1420570 and DMR-2011754).

Author information

Authors and Affiliations



K.K.P. and P.D. supervised the research. K.K.P., P.D., H.C., J.X. and L.A.M. designed the study. H.C., J.X., Z.A. and M.M.P. conducted the experiments and analysed the data. L.A.M., T.X. and J.F.Z. provided support to perform experiments and data analysis. All authors discussed the results and contributed to the writing of the final manuscript.

Corresponding authors

Correspondence to Philip Demokritou or Kevin Kit Parker.

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Competing interests

Harvard University filed for intellectual property relevant to this manuscript, listing K.K.P., P.D., H.C. and L.A.M. as inventors. The remaining authors declare no competing interests.

Peer review

Peer review information

Nature Food thanks Feng Jiang, Long Yu and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Information

Supplementary Figs. 1–8, Table 1 and description of Supplementary Videos 1 and 2.

Reporting Summary

Supplementary Data 5

The effect of antimicrobial pullulan fibre’s surface density on the weight loss of avocados.

Supplementary Data 6

The effect of antimicrobial pullulan fibre coating on avocados’ natural microflora.

Supplementary Data 7

The effect of antimicrobial pullulan fibre coating on the exocarp discoloration of avocados.

Supplementary Video 1

Direct coating avocados with pullulan-based fibres.

Supplementary Video 2

Pullulan fibre coating on avocado was removed by rinsing in water.

Source data

Source Data Fig. 2

Unprocessed data of fibre diameter distribution, X-ray diffraction and Fourier transform infrared spectroscopy.

Source Data Fig. 3

Unprocessed data of antimicrobial and antifungal activity.

Source Data Fig. 4

Unprocessed data of avocado weight and natural microflora (including total aerobic bacteria, yeast and mould).

Source Data Fig. 5

Unprocessed data of avocado’s colour, firmness and pH.

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Chang, H., Xu, J., Macqueen, L.A. et al. High-throughput coating with biodegradable antimicrobial pullulan fibres extends shelf life and reduces weight loss in an avocado model. Nat Food 3, 428–436 (2022).

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