Fig. 4: Transformation of corn with genes expressing the AgThG11 antibody. | Nature Food

Fig. 4: Transformation of corn with genes expressing the AgThG11 antibody.

From: Improved performance of Eimeria-infected chickens fed corn expressing a single-domain antibody against interleukin-10

Fig. 4

a, A map of the transformation cassettes used to express AgThG11 in maize. RB, Agrobacterium T-DNA right border sequence; ZmZ27P, maize 27kDagamma zein promoter; Z27ss, signal sequence from the maize 27kDagamma zein gene; AgThG11:KDEL, coding sequence for AgThG11 with carboxy-terminal KDEL endoplasmic reticulum retention signal; NosT, transcriptional terminator from the Agrobacterium nopaline synthase gene; OsGluP, rice glutelin B-4 promoter; ZmUbi1P, maize ubiquitin 1 promoter with first intron; PMI, phosphomannose isomerase (selectable marker) coding sequence; LB, Agrobacterium T-DNA left border sequence. b, Greenhouse-grown maize plants expressing AgThG11 in seed develop normally.

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