Effects of temperature exposure on the binding affinity of the sdAb AgThG11 (left) and an IgG pAb (right) to cIL-10. Both antibodies were heated to 37 °C, 75 °C, 85 °C or 95 °C in a PCR thermocycler for 20 s, 3 min or 30 min. After 20 min renaturation at room temperature, antibodies were placed on ice. The ELISA (optical density at 450 nm (OD450 nm)) values of the antibody binding affinity to cIL-10 are expressed as the percentage of the binding affinity in an unheated sample. The results are presented as the means of n = 3 independent experiments with standard deviations.