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A safety guide for transgenic Cre drivers in metabolism

Nature Metabolism volume 6, pages 1649–1652 (2024)Cite this article

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Despite the high utility and widespread use of Cre driver lines, lack of Cre specificity, Cre-induced toxicity or poor experimental design can affect experimental results and conclusions. Such pitfalls must be considered before embarking on any Cre-based studies in metabolic research.

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Fig. 1: Workflow and key points to consider for selecting or creating Cre driver lines and their use in experiments.

References

  1. Luo, L. et al. Neuron 106, 37–65.e5 (2020).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Vooijs, M., Jonkers, J. & Berns, A. EMBO Rep. 2, 292–297 (2001).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  3. Lee, J.-Y. et al. J. Biol. Chem. 281, 2649–2653 (2006).

    Article  PubMed  CAS  Google Scholar 

  4. Rashbrook, V. S., Brash, J. T. & Ruhrberg, C. Nat. Cardiovasc. Res. 1, 806–816 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Jeffery, E. et al. Adipocyte 3, 206–211 (2014).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  6. Kim, K. et al. Mol. Metab. 84, 101948 (2024).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Ye, R. et al. Mol. Metab. 4, 771–778 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Moutier, R., Tchang, F., Caucheteux, S. M. & Kanellopoulos-Langevin, C. Transgenic Res. 12, 369–373 (2003).

    Article  PubMed  CAS  Google Scholar 

  9. Moullan, N. et al. Cell Rep. 10, 1681–1691 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  10. Hwang, I. et al. FASEB J. 29, 2397–2411 (2015).

    Article  PubMed  CAS  Google Scholar 

  11. Brinster, R. L., Chen, H. Y., Trumbauer, M. E., Yagle, M. K. & Palmiter, R. D. Proc. Natl Acad. Sci. USA 82, 4438–4442 (1985).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  12. Woychik, R. P. & Alagramam, K. Int. J. Dev. Biol. 42, 1009–1017 (1998).

    PubMed  CAS  Google Scholar 

  13. Yang, H. et al. Cell 154, 1370–1379 (2013).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Kim, J. S. et al. Immunity 54, 176–190.e7 (2021).

    Article  PubMed  CAS  Google Scholar 

  15. Weng, W., Liu, X., Lui, K. O. & Zhou, B. Trends Cell Biol. 32, 324–337 (2022).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland

    Carla Horvath & Christian Wolfrum

  2. Center for Transgenic Models, University of Basel, Basel, Switzerland

    Pawel Pelczar

Authors
  1. Carla Horvath
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  2. Christian Wolfrum
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  3. Pawel Pelczar
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Contributions

C.H., C.W. and P.P. conceptualized, wrote, edited and revised the manuscript. C.H. and P.P. designed the workflow with advice from C.W.

Corresponding authors

Correspondence to Carla Horvath, Christian Wolfrum or Pawel Pelczar.

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The authors declare no competing interests.

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Horvath, C., Wolfrum, C. & Pelczar, P. A safety guide for transgenic Cre drivers in metabolism. Nat Metab 6, 1649–1652 (2024). https://doi.org/10.1038/s42255-024-01087-8

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