Subcellular quantitative analysis has been a long-standing goal of mass spectrometry imaging, but was originally thought to be unattainable. However, recent advances have made organelle-level absolute quantification through mass spectrometry imaging a reality, thanks to the development of nano secondary ion mass spectrometry.
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Siuzdak, G. Subcellular quantitative imaging of metabolites at the organelle level. Nat Metab 5, 1446–1448 (2023). https://doi.org/10.1038/s42255-023-00882-z