Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Subcellular quantitative imaging of metabolites at the organelle level

Subcellular quantitative analysis has been a long-standing goal of mass spectrometry imaging, but was originally thought to be unattainable. However, recent advances have made organelle-level absolute quantification through mass spectrometry imaging a reality, thanks to the development of nano secondary ion mass spectrometry.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type



Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Organelle-level analysis at the sub-50 nm level, allowing MSI of cellular organelles, is now routinely possible.


  1. Caprioli, R. M., Farmer, T. B. & Gile, J. Anal. Chem. 69, 4751–4760 (1997).

    Article  CAS  PubMed  Google Scholar 

  2. Becquart, C. et al. Anal. Chem. 94, 10549–10556 (2022).

    Article  CAS  PubMed  Google Scholar 

  3. Wedlock, L. E. et al. Chem. Commun. 49, 6944–6946 (2013).

    Article  CAS  Google Scholar 

  4. Jiang, H. et al. Chem. Commun. 53, 1506–1509 (2017).

    Article  CAS  Google Scholar 

  5. He, C., Fong, L. G., Young, S. G. & Jiang, H. J. Investig. Med. 65, 669–672 (2017).

    Article  PubMed  Google Scholar 

  6. Legin, A. A. et al. Chem. Sci. (Camb.) 5, 3135–3143 (2014).

    Article  CAS  Google Scholar 

  7. Lovrić, J. et al. ACS Nano 11, 3446–3455 (2017).

    Article  PubMed  Google Scholar 

  8. Thomen, A. et al. ACS Nano 14, 4316–4325 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Nguyen, T. D. K. et al. ACS Nano 16, 4831–4842 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kay, E. et al. Pharmaceuticals 14, 463–470 (2022).

    Article  CAS  Google Scholar 

  11. Palmgren, H. et al. Diabetes (2022).

    Article  PubMed  Google Scholar 

  12. de Boer, P., Hoogenboom, J. P. & Giepmans, B. N. G. Nat. Methods 12, 503–513 (2015).

    Article  PubMed  Google Scholar 

  13. Lovrić, J. et al. Anal. Chem. 94, 12798–12806 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  14. Xu, C. et al. Nature 617, 798–806 (2023).

    Article  CAS  PubMed  Google Scholar 

  15. Northen, T. R. et al. Nature 449, 1033–1036 (2007).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Gary Siuzdak.

Ethics declarations

Competing interests

The author declares no competing interests.

Peer review

Peer review information

Nature Metabolism thanks the anonymous reviewers for their contribution to the peer review of this work.

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Siuzdak, G. Subcellular quantitative imaging of metabolites at the organelle level. Nat Metab 5, 1446–1448 (2023).

Download citation

  • Published:

  • Issue Date:

  • DOI:


Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research