Endothelial cell migration is indispensable for (tumour) angiogenesis and is fuelled by glycolysis. The metabolic underpinnings have been only partially unravelled to date, partly because of the lack of appropriate tools to analyse metabolic flux at the single-cell level. In this issue, Wu et al. introduce a novel imaging assay to capture glycolytic flux during motion in single endothelial cells. With this new tool, the authors uncovered a glycolysis-driven cytoskeletal remodelling apparatus that propels endothelial cell motility.
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P.C. is supported by grants from Methusalem funding (Flemish government), the Fund for Scientific Research-Flanders (FWO-Vlaanderen) and the European Research Council (ERC Advanced Research Grant EU- ERC743074); L.M.B. is supported by a Marie-Curie fellowship. The authors thank M. Dewerchin for help with the manuscript.
P.C. is named as an inventor on patent applications claiming subject matter related to results described in this article. The other authors declare no competing interests.
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Subramanian, A., Becker, L.M. & Carmeliet, P. Endothelial metabolism going single. Nat Metab 3, 593–594 (2021). https://doi.org/10.1038/s42255-021-00399-3