Cell culture media are typically selected on the basis of common laboratory practices but have major effects on the validity, reproducibility and physiological relevance of the scientific findings. We provide arguments and quantitative examples of why choosing an appropriate cell culture medium matters, particularly in metabolic studies.
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Acknowledgements
T.S. has received funding from European Research Council, ERC grant agreement no. 714738.
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S.L. performed the metabolomics data analysis. S.L., E.G. and T.S. wrote the manuscript.
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Lagziel, S., Gottlieb, E. & Shlomi, T. Mind your media. Nat Metab 2, 1369–1372 (2020). https://doi.org/10.1038/s42255-020-00299-y
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DOI: https://doi.org/10.1038/s42255-020-00299-y
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