Fig. 2: Schematic of the de novo assembly of A. marina genome. | Communications Biology

Fig. 2: Schematic of the de novo assembly of A. marina genome.

From: A reference-grade genome identifies salt-tolerance genes from the salt-secreting mangrove species Avicennia marina

Fig. 2

Long-read and short-read data from PacBio Sequel and Illumina, respectively, were subjected to hybrid assembly to build contigs that were combined to build scaffolds. Super-scaffolds were built by combining the scaffolds from Bionano assembly and those from the previous step. Chromosome-scale assembly was built using the Hi-C chromosome conformation capture data, and the assembly was further refined by gap closing and error correction. Sequence data from a scaffold that contained the organellar genomes were re-assembled to build full-length circular chloroplast and mitochondrial genomes.

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