Fig. 2: MMβA-mono binds to the catalytic A-subunit, but not to the B-subunit pentamer, to inhibit Stx. | Communications Biology

Fig. 2: MMβA-mono binds to the catalytic A-subunit, but not to the B-subunit pentamer, to inhibit Stx.

From: Identification of a peptide motif that potently inhibits two functionally distinct subunits of Shiga toxin

Fig. 2

a MMβA-mono efficiently inhibited the cytotoxicity of Stx1a and Stx2a. Data are presented as a percentage of the control value (mean ± SE, n = 3). b AlphaScreen assay to examine the binding between MMβA-tet/MMβA-mono and the A-subunit (upper panels) or the B-subunit pentamer (lower panels). Data are presented as a percentage of the maximum binding value (mean ± SE, n = 7). c Enzyme-linked immunoassay to examine the binding between MMβA-tet/MMβA-mono and the B-subunit pentamer. Data are presented as an Optical Density at 490 nm (OD490) (mean ± SE, n = 3). ***P < 0.001, **P < 0.01, *P < 0.05 by Student’s t test.

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