a Plot of cell area against ROCK1 immunofluorescence for WT, ZO-1,-2 DKO and MAGI-1,-3 DKO cells. Representative images were shown previously in Figs. 1h and 2f. Maximum intensity projections of apical sections determined by α-catenin enrichment were segmented. Data were from ten contiguous cells for each condition. b Plot of cell area against ROCK1 immunofluorescence from data in F collated with additional measurements. Solid line is the interpolated linear regression with the 95% confidence intervals shown by the dotted lines. c Representative immunofluorescence images of WT, ZO-1,-2 DKO, MAGI-1,3 DKO and ASPP1,2 DKO cells stained for NMIIB. Orthogonal views of the cross-section and insets indicated by the white dashed lines and boxes, respectively are shown below. Arrowheads show well-developed NMIIB filaments alongside the junctional actin network. Brackets show NMIIB expansion to the subapical region. Scale bar, 10 μm (inset, 2 μm). d Proposed model for normalization of intercellular tension in the epithelial cell sheet by MAGIs. Source data are available in Supplementary Data 1.