Fig. 4: Par-3 recruitment to AJC is insufficient to downregulate ROCK in the absence of ASPP2-PP1 interaction. | Communications Biology

Fig. 4: Par-3 recruitment to AJC is insufficient to downregulate ROCK in the absence of ASPP2-PP1 interaction.

From: MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis

Fig. 4

a Representative immunofluorescence images of GFP-ASPP2-WTres and GFP-ASPP2-KARAres cells stained for Par-3 (magenta). Scale bar, 10 μm. b Pseudocolor representations of apical areas in GFP-ASPP2-WTres and GFP-ASPP2-KARAres cells. c Violin plot depiction of apical areas in GFP-ASPP2-WTres and GFP-ASPP2-KARAres cells. Data were collated from six measurements obtained over three independent experiments. Lines represent the median and the upper and lower quartiles. P value from unpaired t test is shown. n = 149 (WTres) and 139 (KARAres). d Frequency distributions of apical areas in GFP-ASPP2-WTres and GFP-ASPP2-KARAres cells. Data were as in c. Points are means and error bars are SDs. e P values of D’Agostino & Pearson test for normal distribution were computed from the data presented in d. Medians are notated and shown graphically as lines. f Representative immunofluorescence images of GFP-ASPP2-WTres and GFP-ASPP2-KARAres cells stained for ROCK1. Scale bar, 10 μm. g Cross-junctional line scans of ROCK1 immunofluorescence from images corresponding to f. Shaded area represents AJC as defined by GFP fluorescence. Individual data from 20 independent line scans are shown with the means depicted by solid lines. h Representative immunofluorescence images of GFP-ASPP2-WTres and GFP-ASPP2-KARAres cells stained for aPKC. 3D projections of the region indicated by the yellow dotted square are shown to the right. Scale bar, 10 μm. i Cross-junctional line scans of aPKC immunofluorescence from images corresponding to h. Shaded area represents AJC as defined by GFP fluorescence. Individual data from 20 independent line scans are shown with the means depicted by solid lines. Source data are available in Supplementary Data 1.

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