a Representative images of CNV lesions after different combinations of AIBP and apoA-I treatment. b, Quantification of CNV areas in a. N = 23 (BSA control), 19 (apoA-I), 23 (AIBP), and 20 (AIBP + apoA-I) laser spots. c, d Comparison of AIBP/apoA-I and an anti-VEGF antibody in inhibiting laser-induced CNV in 6–8 weeks (c) and 8-month (d) mice. N = 29 (control), 30 (AIBP + apoA-I), and 24 (anti-VEGF) laser spots in c, and 37 (control), 47 (AIBP + apoA-I), and 43 (anti-VEGF) laser spots in d. e Quantification on the effect of anti-VEGF (low, 5 ng), anti-VEGF (high, 25 ng), AIBP + apoA-I, and AIBP + apoA-I + anti-VEGF (low) in suppressing laser-induced CNV in 18-month male mice. N = 43 (control), 15 (anti-VEGF low), 26 (anti-VEGF high), 19 (AIBP + apoA-I), and 20 (AIBP + apoA-I + anti-VEGF low) laser spots. f Macrophage depletion by Cl2MDP in old mice restored CNV sensitivity to anti-VEGF treatment and blunted the synergistic effect of combination therapy. N = 23 (control), 24 (Cl2MDP), 16 (Cl2MDP+anti-VEGF low), 24 (Cl2MDP+AIBP + apoA-I + anti-VEGF low) laser spots. Data represent mean ± SEM. NS, p > 0.05. Statistical analysis was performed by one-way ANOVA with Tukey post hoc analysis.