Fig. 5: AIBP expression in human CNV, non-lesion, and normal control retinas detected by RNAscope. | Communications Biology

Fig. 5: AIBP expression in human CNV, non-lesion, and normal control retinas detected by RNAscope.

From: Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice

Fig. 5

a, c H&E staining of non-lesion and CNV areas, respectively. b, d, e Representative RNAscope images of non-lesion, CNV, and normal retinas, respectively, show AIBP expression and localization. a and c are adjacent sections for b and d, respectively. f, g, h Magnified image of dashed orange box in b, d, and e, respectively. i, j, k Quantification of AIBP mRNA in Cho-RPE, photoreceptors, and inner retina, respectively. N = 5, 4, and 4 retinas for CNV, non-lesion areas, and normal, respectively. Data represent mean ± SEM. NS, p > 0.05. Statistical analysis was performed by one-way ANOVA with Tukey post hoc analysis. Yellow arrows, orange arrowheads, and orange arrows indicate AIBP expression in the inner segment, RPE, and choroid, respectively, in the normal and non-lesion areas. Black arrowheads and the black arrow indicate AIBP expression in the RPE and choroid, respectively, in normal and CNV areas. Asterisk (*) indicates subretinal disciform scar in CNV. Cho choroid, ROS rod outer segment, RIS rod inner segment, ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer. Scale bar, 20 µm in ae, 10 µm in fh.

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