a Effects of Act5C knockdown on the climbing ability of flies on smooth substrates. The graph illustrates the time taken to climb the vertical glass wall of a Pasteur pipette from the bottom to the constriction (8 cm) (dot plots on the left and the average times (sec) ± SD on the right in each column). The data were analyzed using the Tukey–Kramer test for all pairwise comparisons: P-values are shown on the top. b The panel depicts the percentage of flies capable of overcoming the constriction point on the Pasteur pipette in 1 min. Kruskal–Wallis test, Scheff’s post hoc: **P < 0.001 (P-values are shown on the top). The flies used were of genotypes +; + (CS strain), +/+; svp-GAL4/+, UAS-Act5C RNAi/+; elav-GAL80/+ and UAS-Act5C RNAi/+; svp-GAL4/elav-GAL80. The number of flies examined is shown in parentheses on the x axis. c, d Snapshots of wild-type CS and Act5C knockdown flies (svp > Act5C RNAi, elav-Gal80) climbing the walls of Pasteur pipettes, respectively.