Fig. 4: SMSN reconstruction of immune-labeled TOM20 protein in COS-7 cells. | Communications Biology

Fig. 4: SMSN reconstruction of immune-labeled TOM20 protein in COS-7 cells.

From: Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization

Fig. 4

a Lateral view of SMSN reconstruction from PR-4Pi method. b, c 200-nm thick xz and xy slices of one mitochondria in a (white box), reconstructed from PR-4Pi and contrast methods. d, e Selected single clusters in the xy plane and xz plane and their profiles along the central line, obtained by averaging the pixel values within the white box along the orthogonal dimension of the central line. Each profile was fitted by a 1D Gaussian with a width of \(\sigma\), and the FWHM was calculated from 2.355\(\sigma\). The sample was imaged at a single-optical section with 180,000 frames under epi illumination. Scale bars: 2 µm in a, 200 nm in bd, 50 nm in e, f.

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