Fig. 5: Characterization of cassiosome ultrastructure. | Communications Biology

Fig. 5: Characterization of cassiosome ultrastructure.

From: Cassiosomes are stinging-cell structures in the mucus of the upside-down jellyfish Cassiopea xamachana

Fig. 5

af Individual cassiosome fixed and labeled with Tubulin Antibody, ActinGreenTM and NucBlueTM, and mounted in 80% glycerol in PBS on glass slides for imaging. Imaging was performed with both DIC and confocal laser scanning with lines at 405, 488, 561, and 640 nm, and collected with a Plan Apo ×100 objective. a DIC reveals peripheral layer of nematocytes bearing spherical O-isorhiza nematocysts (lavender arrows). Tubulin (red) reveals cnidocils (short filaments marked by white arrows) extending from apex of nematocytes, and motile cilia (long filaments) originating from non-nematocytes ectoderm cells organized in patches along the peripheral layer among nematocyte-rich areas. NucBlue (blue) reveals nuclei (yellow arrows) of peripheral epithelial layer – nematocytes and other ciliated ectoderm cells. Actin (green) reveals actin basket (pink arrows) formed around the apex of nematocysts. b 3-D construction of Z-stack magnified confocal images corresponding to (a) and (cf) shows tubulin (red) of cnidocils (short filaments marked by white arrows) extending from around apex of nematocytes and motile cilia (long filaments) originating from non-nematocytes putative ectoderm cells organized in patches along the peripheral layer among nematocyte-rich areas. NucBlue (blue) reveals nuclei of peripheral epithelial layer—nematocytes and other ciliated ectoderm cells. Actin (green) reveals actin basket forming around the apex of nematocysts. Scale bars = 10 μm.

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