Fig. 1: The spatial RNA Integrity Number (sRIN) assay. | Communications Biology

Fig. 1: The spatial RNA Integrity Number (sRIN) assay.

From: The spatial RNA integrity number assay for in situ evaluation of transcriptome quality

Fig. 1

a Schematics of an one cell layer thick tissue section placed on a pre-printed fully coated capture area for 18S rRNA, leading to the formation of a c18RNA-footprint. b Schematic depiction of the sequential probe hybridization. c Total RNA gel electropherogram of a mouse olfactory bulb tissue sample together with d a heat map visualization of the section’s spatial RNA integrity number (sRIN) distribution based on its c18RNA footprint (n = 4, technical replicates) and e a HE image of the same section (scale bar: 600 µm), boxes enclose regions shown in close-up. f Close-up of HE image (scale bar: 200 µm) and g sRIN heat map, arrows marks cells from different areas in the tissue with high sRIN values, the left box depicts a group of cells where cell drop-out has occurred compared to the right box that depicts a group of cells recording high sRIN values.

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