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Late Campanian fossil of a legume fruit supports Mexico as a center of Fabaceae radiation


Fabaceae is one of the most diverse angiosperm families and is distributed across the globe in a variety of environments. The earliest evidence of the family, previous to this work, was from Paleogene sediments where it was found to be diverse in many fossil assemblages around the world. Here, we describe a fossil legume fruit from the Olmos Formation (upper Campanian) in northern Mexico. We designated the fossil fruit as Leguminocarpum olmensis Centeno-González, Martínez-Cabrera, Porras-Múzquiz et Estrada-Ruiz sp. nov., and related it with the Fabaceae family based on the presence of a dehiscent pod with two valves, an apex bearing stylar base, short stipe, and reticulated veins in the pericarp. We propose a new fossil species of Leguminocarpum for this fossil fruit. This fossil provides critical information on the long geologic history of Leguminosae around the world, significantly extending the record into the Cretaceous of Mexico.


The legume family is the result of one of the most spectacular radiations of flowering plants. In terms of the number of species, Fabaceae is the third most diverse family, only behind Asteraceae and Orchidaceae, including ca. 730 genera and ca. 19,400 species1,2, and represents one of the most ecologically diverse groups3. The Fabaceae is widely distributed throughout the world, especially in biomes such as the tropical rainforests and the dry forests of America.

Until now, there were no fossils fruits unequivocally belonging to the Fabaceae before the Paleogene4. However, by the Paleocene, the family was already diverse in many fossil assemblages around the world4,5,6,7,8,9. In North America, legume fossils are known beginning around 65.35 mya9. These records include genera found in current warm-temperate forests in the southeastern United States, but also genera restricted to the present day tropics5. The Fabaceae fossil record in Mexico is extensive and includes a wealth of vegetative and reproductive organs10,11,12,13,14,15,16.

In this paper, we described a fossil fruit of a legume from the upper Campanian in northern Mexico. The fruit has a combination of characters that relates it to different Fabaceae subfamilies such as Cercidoideae, Detarioideae, Caesalpinioideae and Papilionoideae. The legume family has a rich fossil record around the world, especially in Eocene and younger sediments, but this fossil significantly extends the record into the Cretaceous of Mexico.

Results and discussion

Systematic description.


Genus—Leguminocarpum Dotzler

Species—Leguminocarpum olmensis sp. nov. Centeno-González, Martínez-Cabrera, Porras-Múzquiz et Estrada-Ruiz


The specific epithet refers to the Olmos Formation, where the fossil was collected.

Fossil material

Holotype MUZ-3907 (Figs. 13).

Fig. 1: Fossil fruit from the Olmos Formation, Holotype MUZ-3907.

a General view of the fossil specimen, showing stylar base (upper arrow), and fracture marks (bottom arrows). b A partially opened suture (upper arrow), and patterns of venation (bottom arrow).

Fig. 2: Line-drawing of Holotype (MUZ-3907).

Reconstruction of the fossil fruit, showing fracture lines in the frontal valve, some veins in the epicarp, as well as the suture by which the valves are partially joined. Dotted lines represent areas where the fruit is partially visible.

Fig. 3: Detail of the fossil sample, Holotype MUZ-3907.

a Showing of the apex of fruit. b Base of the fruit, preserving reticulated veins (white arrow), and the stipe (black arrow). c Suture (black arrow), and fracture marks (white arrows).


Olmos Formation (73.5 mya). Tajo La Florida, near the town of Melchor Múzquiz, Coahuila, with coordinates 27° 39′ 32.1″ N, -101° 19′ 10.7″ W.


Upper Campanian, Olmos Formation.

Place of deposit

Museo de Paleontología de Múzquiz, Melchor Múzquiz, Coahuila, Mexico.

Species diagnosis

Legume fruit, asymmetric, curved shape; 3–4 times longer than wide; apex rounded, right angled, bearing a stylar base; base rounded to tapered, right-angled; short stipe; compressed; dehiscent along both sutures; without visible chambers, epicarp glabrous, reticulated veins, with ribs; wingless.


The description is based on one specimen (Figs. 13). The fossil preserves a partial oblique view and therefore it is possible to distinguish both valves joined by a suture. The fruit is strongly asymmetrical, with curved shape. Both sutures parallelly curved without twists; the partially opened suture is thick (Figs. 13). Margin not constricted. The fruit is 3 to 4 times longer than wide, the length of the fruit is 4.55 cm, and the maximum width of the valve is 1.25 cm; the smallest width near both the apex and base is 0.9 cm. The fruit is at least partially dehiscent. The two valves have separated in the apical part of the fruit and have shifted laterally, the style base appears on each valve (Figs. 13a). The apex is rounded and likely aligned to the partially opened suture, bearing a stylar base (Figs. 1a–3a). Base shape is rounded to tapered and right-angled. The fruit preserved the stipe with 4 mm long, both receptacle and pedicel total measure is 6.5 mm in length and 2.5 mm in diameter (Fig. 3b). The fruit is compressed, and does not have visible chambers. The fossil preserves numerous veins that arise from the sutures, forming reticulated veins closely spaced, oriented at an angle of approximately 90° relative to the dorsal and ventral sutures (Figs. 1b, 2, 3b, c). The valves have fracture marks, perpendicularly oriented to both sutures (Figs. 1a, 2, 3c).

Taxonomic commentaries

The fossil fruit from the Olmos Formation was compared with Lardizabalaceae, Ranunculaceae, Apocynaceae, Proteaceae, Bignoniaceae, Annonaceae, and Fabaceae families. The fruits of Lardizabalaceae have fleshy follicles, dehiscent or indehiscent, and their shape elongate-oblong or subglobose. Examples of fruits with a possible morphological resemblance with the fossil in Lardizabalaceae were Decaisnea Hook. f. and Thomson and Holboellia Wall. Decaisnea has, however, features not present in the fossil such as woody epicarp, globose, and dehiscence along one suture. Holboellia, in addition, further differentiates from the fossil in having tuberculose epicarp, and a thick stipe. Despite these general similarities in shape and apex, the globose valves fruits, and the dehiscence along one suture prevents the inclusion of the fossil in the family.

Fruits in Ranunculaceae include achenes, berries, or follicles. Among the genera with some similarity with the fossil were Helleborus L., Delphinium L., Aquilegia L., and Actea L. Among the few similarities shared with the fossil, were the glabrous epicarp and the apex with a large beak. Because of the dehiscence along only one suture, differences in the general form, as well as the high prevalence of achenes in Ranunculaceae, L. olmensis bears no affinity with this family.

Fruits in Apocynaceae have some features in common with the fossil, specifically with Gonolobus Michx., Cynanchum L., Matelea Aubl., Calotropis R. Br., and Tabernaemontana L. Gonolobus has right-angled apex and base, large tapering, and conspicuously angled sutures. Cynanchum is smooth, lanceolate-ovoid in shape, 11–16 cm in length, thus setting it apart from the fossil. Matelea has fruits 9–11 cm in length and epicarp muricate. Calotropis has large fruits, 6–12 cm in length and 3–7 cm in width, rounded-ovate in shape, sub-globose and bladdery. Tabernaemontana has globose, straight-curve or rounded fruit, dehiscent along one suture. Although some sets of features present in Apocynaceae fruits resemble the fossil, they are different in size (up to 5 cm), the tubercular or or longitudinal ribs in the epicarp, and globose shape in transection, with curve-rounded shape, or curve with apex large with a beak, and thus we rule out any affinity.

Fruits in the Proteaceae family are fleshy, or non-fleshy, woody, with the carpel dehiscent along one suture, or indehiscent, there are follicles, drupes, or achenes. The follicles have similarities with the fossil only in the apex features, as well as in the presence of the stipe. However, the epicarp features, the globose valves (such as in Telopea R. Br., Xylomelum Smith and Sm., Persoonia Sm., Hackea Schrad. and J.C.Wendl., and Grevillea R. Br. ex Knight), and the general shape on fruit were distinct from the fruits from the Olmos Formation.

Bignoniaceae has fruits with two valves, but differs with the fossil in having linear or lanceolate or rounded shape, as well as the presence of a stronger longitudinal vein parallel to the valves, or a glabrous epicarp without veins. Many of these fruits with valves are linear or lanceolate in shape, being longest than wide, this is the case of Bignonia L., Tabebuia Gomes ex DC., Pyrostegia C. Presl, Lundia DC., Dolichandra Cham., Fridericia Mart., Mansoa DC., Tanaecium Sw., Campsis Lour., and Handroanthus Mattos. In some Bignoniaceae fruits the epicarp were pubescent or woody. Other fruits are distinct form than linear or lanceolate, such is the case of Amphitecna Miers, Anemopaegma Mart. ex Meisn., Amphilophium Kunth, Jacaranda Juss., Pithecoctenium Mart. ex Meisn., and Kigelia DC. Amphitecna has a curve-ovate shaped, non-stipate, rounded or short tapered-truncate base, globose, with 1 stronger longitudinal vein parallel to the valves, and glabrous in epicarp, without veins. Anemopaegma is tapered in base and apex, large beaked, stipate, and dehiscent, with a parallel line along the sutures. Amphilophium is subterete, with curve shape, two times longer than wide, short tapered base and substipate, ligneous epicarp without veins, and multiple seeds. Jacaranda is rounded in shape, compressed, bearing stylar base, rounded in base and apex, with longitudinal line parallel to valves, non-stipate. Pithecoctenium has a stylar base, nevertheless is a non-stipate fruit, with epicarp spinose, and globose transection. Anemopaegma is substipate fruit, short tapered in the base, and has a longitudinal line parallel to valves, the epicarp is coriaceous and glabrous. Kigelia is a curve, globose fruit, woody in epicarp, non-stipate, with rounded base and apex. Because of these differences we concluded the fossil is not related to this family.

Anonnaceae has some species with two valves and some genera bear stylar base. Nevertheless the fruits in this family are indehiscent, fleshy and globose shape in transection, the epicarp is glabrous, tuberculose, pubescent or spinose, without veins, being non-stipate or bearing a thick stipe. These characteristics are not present in the fossil fruit and are evident in genera such as Xylopia L., Meiocarpidium Engl. and Diels, Orophea Blume, Monanthotaxis Baill., Uvaria L., Cymbopetalum Benth., Klarobelia Chatrou, and Uvariopsis Engl.

Lastly, the most notable characteristics present in fruits belonging to all legume subfamilies are a single superior carpel with one locule, marginal placentation, one to many ovules in two alternating rows on a single placenta, and dehiscent or indehiscent pods2,17 (Supplementary Data 1). The main features in common among the legume genera more similar to Leguminocarpum olmensis were the proportion, length, and width of the fruit, compressed transection, stylar base preserved, not visible chamber, the epicarp features, and the absence of a wing. Other features shared between the fossil and the reviewed genera were base form, apex form, the shape of the fruit, length of the stipe, and type of dehiscence. In particular, the fossil fruits have characteristics present in extant fruits of Cercidoideae, Detarioideae, Caesalpinioideae and Papilionoideae subfamilies (Fig. 4, Supplementary Data 1), from these subfamilies, some extant genera sharing more features with the fossil fruits are Calpocalix harms, Macrosamanea Britton, Rose ex Britton and Killip, Microlobius foetidus (Jacq.) Sousa and G., Griffonia Baill., Colophospermum Kirk ex Léonard, Baphiopsis Benth. ex Baker, Baptisia Vent., Bossiaea Vent., Bowdichia Kunth, Dalbergia L.f., Haplormosia Harms, Harpalyce Moç., and Sessé ex D. C., Isotropis Benth. Despite the resemblance between L. olmensis and extant Fabaceae, it could not be placed in any subfamily, because the fossil shows features morphologically similar to four extant subfamilies (see Supplementary Data 1).

Fig. 4: Extant samples of Fabaceae.

a Cynometra oaxacana Brandegee (Detarioideae: 1509871–MEXU). b Peltogyne mexicana Martínez. (Detarioideae: 1032703–MEXU). c Barnebydendron riedelii (Tul.) J.H. Kirkbr. (Detarioideae: 1003269–MEXU). d Bossiaea rhombifolia Sieber ex DC. (Papilionidae: 469928–MEXU). e Harpalyce arborescens Gray (Papilionidae: 579909–MEXU). f Gymnocladus dioicus (L.) K. Koch. (Caesalpinioideae: 892167–MEXU). g Gleditsia amorphoides (Griseb.) Taub. (Caesalpinioideae: 650165–MEXU). h Microlobius foetidus (Jacq.) M. Sousa and G. (Caesalpinioideae: 1019245–MEXU). i Baptisia bracteata Elliot (Papilionidae: 962897–MEXU). j Baptisia lactea (Rafinesque) Thieret (Papilionidae: 142687–MEXU).

Globally, legume fossil fruits have been described from Cenozoic sediments worldwide4,6,8,9,10,11,12,18,19,20,21,22,23,24,25. The fossil fruits Mezoneuron claibornensis Herendeen and Dilcher, Mezoneuron flumen-viridensis Herendeen and Dilcher, and Mezoneuron spokanensis (Knowlton) Herendeen and Dilcher, share with L. olmensis the presence of an apex bearing stylar base, a tapered base, and the presence of veins in the epicarp21. However, the flat membranous winged samaras, and the short, or absent stipe in these three fossil species preclude any relationship with the fossil fruits from the Olmos Formation. Apuleia herendeenii Calvillo-Canadell and Cevallos-Ferriz shares with L. olmensis the dehiscent valves and lack of visible chambers23. However, L. olmensis has major differences, namely the parallel curvature in both sutures, the curved shape, the rounded to aligned apex, rounded to the tapered base with the stipe, and the configuration of the veins on the valves. Podocarpium podocarpum (Braun) Herendeen (Supplementary Data 1) shows features in common to the fossil fruits such as a glabrous with obliquely reticulate venation, without visible chambers, as well as the length of the stipe, and the apex form; nevertheless P. podocarpum presents other different characteristics such as in the size fruit, symmetric form of the fruit, and the base form. Recently, several Paleocene fruits belonging to Detarioideae have been described8, among the specimens described, the fossil fruit morphotype 8 shows some characteristics shared with the fossil fruits from the Olmos Formation such asymmetry, lack of wings, stipe, and stylar base. The morphotype 8, however, has an acute tapered base, obtuse-acute apex and epicarp venation straight and oblique8 (Supplementary Data 1). Other fossil fruits have some features restricting the resemblance to L. olmensis, this is the case of Mezoneuron claibornensis Herendeen and Dilcher19, Crudia Grahamiana Herendeen and Dilcher20, Eliasofructus claibornensis Herendeen and Dilcher18, Prosopis lazarii Magallón-Puebla and Cevallos-Ferriz13, Lysiloma mixtecana Magallón-Puebla and Cevallos- Ferriz13, Mimosa tepexana Magallón-Puebla and Cevallos-Ferriz13, Sophora sousae Magallón-Puebla and Cevallos-Ferriz13, Reinweberia omithopoides Magallón-Puebla and Cevallos-Ferriz13 (Supplementary Data 1).

The fossil fruits from the Olmos Formation show differences to other previously described fossils and extant fruits (Supplementary Data 1), and therefore we propose a fossil species in Leguminosae named Leguminocarpum olmensis Centeno-González, Martínez-Cabrera, Porras-Múzquiz et Estrada-Ruiz.

Biogeographic significance

Because of the high generic diversity of the group in tropical America and Africa-Madagascar, these regions have been suggested, in what it is known as the Gondwana hypothesis, as the place of origin and radiation of Fabaceae during the Upper Cretaceous, when these continents were in close proximity1,4,26. After that period, and throughout the Cenozoic, legumes were thought to have migrated to South America and North America, leaving behind the early divergent genera in Africa26,27,28. The Gondwanan hypothesis, however, is not supported by advances in the understanding of continental drift and the availability of more precise phylogenies for legumes1,29, as well as the reinterpretation of Eocene fossils4,5. This phylogenetic evidence also indicates that many South American records considered in the past as early divergent taxa correspond instead to recent offshoots from the northern hemisphere radiations, in contrast to the Gondwanan hypothesis1. The earliest records of Fabaceae fossils from different regions in the world are taxa previously established towards the Paleocene-Eocene boundary4,6,7,8,9,30,31,32. Their widespread distribution at that time suggests an earlier origin and diversification4,22,29,33. In recent studies, the majority of molecular phylogenies have proposed the origin of the crown group of the family between 59 and 70 (92.1) mya29,34,35,36,37,38. Leguminocarpum olmensis, with an age of ~73.5 Ma, gives support to the age proposed by molecular dating analyses previously cited, pointing to an earlier origin and diversification in the fossil record of Fabaceae, granted that L. olmensis is not a member of the stem group.

In North America, the Leguminosae were abundant and diverse during the Paleogene, suggesting North America as an important region for the evolutionary history of the family4. In Mexico, Leguminosae fossils have been recorded in Cenozoic sediments9,11,39,40. These fossil species recorded in Mexico have been related to both subtropical and tropical extant taxa currently growing in Central and South America41,42,43. Furthermore, in Mexico, the extant family is the second most diverse family just behind Asteraceae, with 1850 extant species belonging to 139 genera44. This record of Leguminocarpum olmensis in northern Mexico significantly extends the presence of Fabaceae into the Cretaceous of Mexico, suggesting low latitude North America as a place for the early evolution of Leguminosae.


The fossil fruit from the Olmos Formation is described as a Cretaceous species of Leguminocarpum in  Fabaceae. The majority of the features present in the fossil can be found in all subfamilies of Fabaceae. However, L. olmensis most closely resembles species in Cercidoideae, Detarioideae, Caesalpinioideae and Papilionoideae subfamilies, and therefore its placement at this taxonomical level is uncertain. Although its within family affinities are unknown, this fossil extends the record of legumes into the Cretaceous of Mexico, suggesting low latitude North America, particularly northern Mexico, as a place for the early evolution of Leguminosae.


Geological setting

The paratropical rainforest of the Olmos Formation (upper Campanian), represents one of the most diverse fossil floras in the Americas45,46,47,48,49,50,51. In the Olmos Formation, there have been identified gymnosperms, such as Cupressaceae52,53, and aquatic ferns including Salvinia, Dorfiella† and Marsilea49,54,55. Among the numerous angiosperm leaves collected in the formation, there are taxa belonging to Arecaceae, Araceae, Moraceae, Betulaceae, Magnoliaceae, Lauraceae, Rhamnaceae, Menispermaceae, Nelumbonaceae, Caprifoliaceae, and Violaceae46,47,49,56,57. In addition, based on permineralized angiosperm woods and stem, there are fossil genera placed in Arecaceae, Malvaceae, Fagaceae, Anacardiaceae, Lauraceae, Cornaceae, Ericales, as well as Metcalfeoxylon47,58,59.

The Olmos Formation is one of the formations in the Navarro Group, which is located in the Sabinas Basin. It represents a fluvial-deltaic system, and based on the study of its lithofacies and fossils, has four depositional sub-environments: (1) lithofacies A, rich in coal, suggesting it corresponds to a swampy area with restricted circulation; (2) lithofacies B, composed of shale and sandstone that may represent floodplain environments and/or lagoons with open circulation; (3) lithofacies C, composed of fine to medium grained sandstone, with organic matter and parallel lamination and representing a fluvial environment, probably braided rivers, as suggested by the geometry of the sandbars and channel fills within the facies, and finally; (4) lithofacies D, composed of cross-stratified sandstones, interpreted as channel facies and levee deposited by a meandering river49,51,57. The fossil fruit described here was collected in the lithofacies B.

The age of the Olmos Formation has been dated as upper Campanian to lower Maastrichtian, starting with planktonic foraminifera assemblages Rosita fornicata/stuatiformis60, and palynology studies61 suggesting Lower Maastrichtian. Some ammonites in situ indicated an Upper Campanian age62. Furthermore, low adjacent San Miguel Formation is considered as Upper Campanian based on indicator fossils63,64, meanwhile, upper adjacent Escondido Formation contains the bivalves Exogyra costata Say and Pycnodonte mutabilis (Morton), giving it the age of Lower Maastrichtian (Personal communication, Vega-Vera, 2006). The age of the formation has been based on detrital zircons collected in sandstones in the lithofacies B of the Olmos Formation, where the fruits were collected, yielded and age of ~73.5 Ma, Upper Campanian (Personal communication, Callejas-Moreno, 2019).

Collection and processing of fossil material

The fossil fruit was collected in 2016 from the locality known as Tajo La Florida, with coordinates 27° 39′ 32.1” N, and 101° 19′ 10.7” W. This locality is a private open mine located northwest of the town of Múzquiz, Melchor Múzquiz municipality, Coahuila, Mexico.

The observations of the morphological characteristics of the fossil fruits, as well as obtain the deep of fruit embedded in the rock, it was taken photographs by slices of the fossil, helped by the Microscope Zeiss AXIO Zoom.V16, and photographed with both AxioCam MRc5 camera and SC100 digital camera 5 Mpix., and the program Zen 2012, blue edition. It was carried out a tomography with CT Scanner Phillips Brilliance, 64-slice, to see some internal structure, nevertheless, none internal structure was observed due to the composition and hardness of the material.

The morphological description was made following terminology provided by specialized literature17,65,66,67,68,69,70. We compared the morphology of the fossil fruit with different families with material from the Herbarium at the Escuela Nacional de Ciencias Biológicas, IPN, and National Herbarium (MEXU), Instituto de Biología, UNAM, and digital herbaria of Missouri Botanical Garden71, and Royal Botanical Garden72. Once obtained a better morphological resemblance between the fossil and the extant specimens from one particular family, we carried a detailed search consulting herbaria data from the Herbarium at the Escuela Nacional de Ciencias Biológicas, IPN, and National Herbarium (MEXU), Instituto de Biología, UNAM, Mexico, specialized literature66,67,69,70,73,74, as well as fossil records from different ages8,13,18,19,20,21,22,23,24,25,69,75,76,77,78. The species with closer similarity to the fossil fruit may are in Supplementary Data 1. Characteristics observed embraced the size of the fruit, general shape, apex, and the base shape, presence, and length of the stipe, transection, dehiscence of valves, visible or not visible chambers, as well as characteristics of pericarp and presence or absence wing. The fossil fruit described in this paper is deposited in the Museo de Paleontología de Múzquiz (MUZ-3907), Melchor Múzquiz, Coahuila, Mexico. This paleontological collection is formally certified by the Instituto Nacional de Antropología e Historia (INAH), which formally protects the Mexican paleontological patrimony.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The authors declare that the data supporting the findings of this study are available in the paper and its Supplementary Data files. The specimen is held in Museo de Paleontología de Múzquiz, México with a specimen number MUZ-3907.


  1. 1.

    Doyle, J. J. & Luckow, M. A. The rest of the iceberg. Legume diversity and evolution in a phylogenetic context. Plant Physiol. 131, 900–910 (2003).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  2. 2.

    Lewis, G. P., Schrire, B., MacKinder, & Lock, M. (eds). Legumes of the World (Royal Botanical Gardens, 2005).

  3. 3.

    McKey, D. Advances in Legume Systematics 5: the Nitrogen Factor. (eds. Sprent, J. I. & Mckey, D.) 211–228 (Royal Botanical Gardens, 1994).

  4. 4.

    Herendeen, P. S., & Dilcher, D. L. (eds) Advances in Legume Systematics, Part 4. The Fossil Record. (Royal Botanic Gardens, 1992).

  5. 5.

    Taylor, D. W. Paleobiogeographic relationships of angiosperms from the Cretaceous and early Tertiary of the North American area. Bot. Rev. 56, 279–415 (1990).

    Article  Google Scholar 

  6. 6.

    Wing, S. L. et al. Late Paleocene fossils from the Cerrejón formation, Colombia, are the earliest record of neotropical rainforest. Proc. Natl Acad. Sci. USA 106, 18627–18632 (2009).

    CAS  PubMed  Article  Google Scholar 

  7. 7.

    Herendeen, P. S. & Herrera, F. Eocene fossil legume leaves referable to the extant genus Arcoa (Caesalpinioideae, Leguminosae). Int. J. Plant Sci. 180, 220–231 (2019).

    Article  Google Scholar 

  8. 8.

    Herrera, F., Carvalho, M. R., Wing, S. L., Jaramillo, C. & Herendeen, P. S. Middle to Late Paleocene Leguminosae fruits and leaves from Colombia. Austral. Syst. Bot. 32, 385–408 (2019).

    Google Scholar 

  9. 9.

    Lyson, T. R. et al. Exceptional continental record of biotic recovery after the Cretaceous–Paleogene mass extinction. Science 366, 977–983 (2019).

    CAS  PubMed  Article  Google Scholar 

  10. 10.

    Miranda, F. Two fossil plants from the amber of Simojovel, Chiapas, México. J. Paleontol. 37, 611–614 (1963).

    Google Scholar 

  11. 11.

    Palacios-Chávez, R. & Rzedowski, J. Estudio palinológico de las floras fósiles del Mioceno inferior y principios del Mioceno medio de la región de Pichucalco, Chiapas, México. Acta Bot. Mex. 24, 1–96 (1993).

    Article  Google Scholar 

  12. 12.

    Cevallos-Ferriz, S. R. S. & Barajas-Morales, J. Fossil woods from the El Cien Formation in Baja California Sur: Leguminosae. Int. Assoc. Wood Anat. J. 15, 229–245 (1994).

    Google Scholar 

  13. 13.

    Magallón-Puebla, S. A. & Cevallos-Ferriz, S. R. S. Fossil legume fruits from tertiary strata of Puebla. Mex. Can. J. Bot. 72, 1027–1038 (1994).

    Article  Google Scholar 

  14. 14.

    Calvillo-Canadell, L. & Cevallos-Ferriz, S. R. S. Bauhcis moranii gen. et sp. nov. (Cercideae, Caesalpinieae), an Oligocene plant from Tepexi de Rodríguez, Puebla, Mexico, with leaf architecture similar to Bauhinia and Cercis. Rev. Palaeobot. Palynol. 122, 171–184 (2002).

    Article  Google Scholar 

  15. 15.

    Poinar, G. O. Jr. & Brown, A. E. Hymenaea mexicana sp. nov. (Leguminosae: Caesalpinioideae) from Mexican amber indicates Old World connections. Bot. J. Linn. Soc. 139, 125–132 (2002).

    Article  Google Scholar 

  16. 16.

    Martínez-Cabrera, H. I., Cevallos-Ferriz, S. R. S. & Poole, I. Fossil woods from the Early Miocene sediments of El Cien Formation, Baja California Sur, Mexico. Rev. Palaeobot. Palynol. 138, 141–163 (2006).

    Article  Google Scholar 

  17. 17.

    Azani, N. et al. A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny. Taxon 66, 44–77 (2017).

    Article  Google Scholar 

  18. 18.

    Herendeen, P. S. & Dilcher, D. L. Fossil Mimosoid Legumes from the Eocene and Oligocene of southeastern North America. Rev. Palaeobot. Palynol. 62, 339–361 (1990).

    Article  Google Scholar 

  19. 19.

    Herendeen, P. S. & Dilcher, D. I. Diplotropis (Leguminosae, Papilionoideae) from the Middle Eocene of Southeastern North America. Syst. Bot. 15, 526–533 (1990).

    Article  Google Scholar 

  20. 20.

    Herendeen, P. S. & Dilcher, D. L. Reproductive and vegetative evidence for the occurrence of Crudia (Leguminosae Caesalpinioideae) in the Eocene of Southeastern North America. Bot. Gaz. 151, 402–413 (1990).

    Article  Google Scholar 

  21. 21.

    Herendeen, P. S. & Dilcher, D. L. Caesalpinia subgenus Mezoneuron (Leguminosae, Caesalpinioideae) from the Tertiary of North America. Am. J. Bot. 78, 1–12 (1991).

    Article  Google Scholar 

  22. 22.

    Crepet, W. L. & Taylor, D. W. The diversification of the leguminosae: first fossil evidence of the Mimosoideae and Papilionoideae. Science 228, 1087–1089 (1985).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  23. 23.

    Calvillo‐Canadell, L. & Cevallos‐Ferriz, S. R. S. Diverse Assemblage of Eocene and Oligocene Leguminosae from Mexico. Int. J. Plant Sci. 166, 671–692 (2005).

    Article  Google Scholar 

  24. 24.

    Wang, H., Blanchard, J. & Dilcher, D. L. Fruits, seeds, and flowers from the Warman clay pit (middle Eocene Claiborne Group), western Tennessee, USA. Palaeontol. Electron. 16, 31A–73A (2013).

    Google Scholar 

  25. 25.

    Jia, H. & Manchester, S. R. Fossil leaves and fruits of Cercis l. (Leguminosae) from the Eocene of Western North America. Int. J. Plant Sci. 175, 601–612 (2014).

    Article  Google Scholar 

  26. 26.

    Raven, P. H. & Polhill, R. M. Advances in Legume Systematics, Part 1 (eds. Polhill, R. M. & Raven, P. H.) 27–34 (Royal Botanic Gardens, 1981).

  27. 27.

    Raven, P. H. & Axelrod, D. I. Angiosperm biogeography and past continental movements. Ann. Mo. Bot. Gard. 61, 539–657 (1974).

    Article  Google Scholar 

  28. 28.

    Morley, R. J. Origin and Evolution of Tropical Rain Forests (John Wiley & Sons, 2000).

  29. 29.

    Koenen, E. J. M. et al. The origin and early evolution of the legumes are a complex paleopolyploid phylogenomic tangle closely associated with the Cretaceous-Paleogene (K-Pg) Boundary. BioRxiv 577957 (2019).

  30. 30.

    Axelrod, D. I. Advances in Legume Systematics, Part 4. The Fossil Record (eds. Herendeen, P. S. & Dilcher, D. L.) 259–279 (Royal Botanic Gardens, 1992).

  31. 31.

    Herendeen, P. S., Crepet, W. L. & Dilcher, D.L. Advances in Legume Systematics, Part 4. The Fossil Record (eds. Herendeen, P. S. & Dilcher, D. L.) 303–316 (Royal Botanic Gardens, 1992).

  32. 32.

    Herendeen, P. S. & Crane, P. R. Advances in Legume Systematics, Part 4. The Fossil Record (eds. Herendeen, P. S. & Dilcher, D. L.) 57–68 (Royal Botanic Gardens, 1992).

  33. 33.

    Crepet, W. L. & Herendeen, P. S. Advances in Legume Systematics, Part 4. The Fossil Record (eds. Herendeen, P. S. & Dilcher, D. L.) 43–55. (Royal Botanic Gardens, 1992).

  34. 34.

    Crepet, W. L., Nixon, K. C. & Gandolfo, M. A. Fossil evidence and phylogeny: the age of major angiosperm clades based on mesofossil and macrofossil evidence from Cretaceous deposits. Am. J. Bot. 91, 1666–1682 (2004).

    PubMed  Article  Google Scholar 

  35. 35.

    Magallón, S. A. & Sanderson, M. J. Absolute diversification rates in angiosperm clades. Evolution 55, 1762–1780 (2001).

    PubMed  Article  PubMed Central  Google Scholar 

  36. 36.

    Magallón, S. A., Gómez-Acevedo, S., Sánchez-Reyes, L. L. & Hernández-Hernández, T. A metacalibrated time-tree documents the early rise of floweringplant phylogenetic diversity. N. Phytologist 207, 437–453 (2015).

    Article  Google Scholar 

  37. 37.

    Lavin, M. & Luckow, M. Origins and relationships of tropical North America in the context of the boreotropics hypothesis. Am. J. Bot. 80, 1–14 (1993).

    Article  Google Scholar 

  38. 38.

    Bruneau, A., Mercure, M., Lewis, G. P. & Herendeen, P. S. Phylogenetic patterns and diversification in the caesalpinioid legumes. Botany 86, 697–718 (2008).

    CAS  Article  Google Scholar 

  39. 39.

    Langenheim, J. Botanical source of amber from Chiapas, Mexico. Ciencia 24, 201–210 (1966).

    Google Scholar 

  40. 40.

    Pérez-Lara, D. K., Estrada-Ruiz, E. & Castañeda-Posadas, C. New fossil woods of Fabaceae from El Bosque Formation (Eocene), Chiapas, Mexico. J. South Am. Earth Sci. 93, 1–13 (2019).

    Article  Google Scholar 

  41. 41.

    Cowan, R. S. & Polhill, R. M. Advances in Legume Systematics, Part 1 (eds. Polhill, R. M. & Raven, P. H.) 117–134 (Royal Botanic Gardens, 1981).

  42. 42.

    Lewis, G. P. & Elias, T. S. in Advances in legume systematics, part 1 (eds. Polhill, R. M. & Raven, P. H.) 155–168. (Royal Botanic Gardens, 1981).

  43. 43.

    Vassal, J. in Advances in legume systematics, part 1 (eds. Polhill, R. M. & Raven, P. H.) 169–171 (Royal Botanic Gardens, 1981).

  44. 44.

    Sousa, M., Ricker, M. & Hernández, H. M. Three species of the family Leguminosae in Mexico. Harv. Pap. Bot. 6, 339–365 (2001).

    Google Scholar 

  45. 45.

    Weber, R. La vegetación Maestrichtiana de la Formación Olmos de Coahuila. México. B. Soc. Geol. Mex. 33, 5–19 (1972).

    Google Scholar 

  46. 46.

    Weber, R. Some aspects of the Upper Cretaceous angiosperms, flora of Coahuila, Mexico. Cour. Forsch. Inst. Senckenberg 30, 38–46 (1978).

    Google Scholar 

  47. 47.

    Estrada-Ruiz, E., Martínez-Cabrera, H. I. & Cevallos-Ferriz, S. R. S. Fossil woods from the Olmos Formation (late Campanian-early Maastrichtian), Coahuila, Mexico. Am. J. Bot. 97, 1179–1194 (2010).

    PubMed  Article  PubMed Central  Google Scholar 

  48. 48.

    Estrada-Ruiz, E., Upchurch, G. R. Jr., Wolfe, J. A. & Cevallos-Ferriz, S. R. S. Comparative morphology of fossil and extant leaves of Nelumbonaceae, including a new genus from the Late Cretaceous of western North America. Syst. Bot. 36, 337–351 (2011).

    Article  Google Scholar 

  49. 49.

    Estrada-Ruiz, E., Centeno-González, N. K., Aguilar-Arellano, F. & Martínez-Cabrera, H. I. New record of the aquatic fern Marsilea, from the Olmos Formation (Upper Campanian), Coahuila, Mexico. Int. J. Plant Sci. 179, 487–496 (2018).

    Article  Google Scholar 

  50. 50.

    Martínez-Cabrera, H. I., Ramírez-Garduño, J. L. & Estrada-Ruiz, E. Plantas fósiles e inferencia paleoclimática: aproximaciones metodológicas y algunos ejemplos para México. B. Soc. Geol. Mex. 66, 41–52 (2014).

    Article  Google Scholar 

  51. 51.

    Estrada-Ruiz, E., Martínez-Cabrera, H. I., Callejas-Moreno, J. & Upchurch, G. R. Jr. Floras tropicales cretácicas del norte de México y su relación con floras del centro-sur de América del Norte. Polibotánica 36, 41–61 (2013).

    Google Scholar 

  52. 52.

    Serlin, B. S., Delevoryas, T. & Weber, R. A new conifer pollen cone from the upper Cretaceous of Coahuila, Mexico. Rev. Palaeobot. Palynol. 31, 241–248 (1981).

    Article  Google Scholar 

  53. 53.

    Hernández-García, I. P. Diversidad taxonómica de coníferas de la Formación San Carlos, Chihuahua y la Formación Olmos, Coahuila del Cretácico Superior del norte de México. Tesis de Maestría, Universidad Nacional Autónoma de México. (2018).

  54. 54.

    Weber, R. Salvinia coahuilensis nov. sp. del Cretácico Superior de México. Ameghiniana 10, 173–190 (1973).

    Google Scholar 

  55. 55.

    Weber, R. Dorfiella auriculata f. gen. nov. sp. nov., un género nuevo de helechos acuáticos del Cretácico Superior de México. Boletín de la Asociación Latinoamericana de Paleobotánica y Palinología 3, 1–13 (1976).

    Google Scholar 

  56. 56.

    Estrada-Ruiz, E., Upchurch, G. & Cevallos-Ferriz, S. R. S. Flora and climate of the Olmos Formation (upper Campanian-lower Maastrichtian), Coahuila, Mexico. A preliminary report. GCAGS 58, 273–283 (2008).

    Google Scholar 

  57. 57.

    Centeno-González, N. K., Porras-Múzquiz, H. & Estrada-Ruiz, E. A new fossil genus of angiosperm leaf from the Olmos Formation (upper Campanian), of northern Mexico. J. South Am. Earth Sci. 91, 80–87 (2019).

    Article  Google Scholar 

  58. 58.

    Estrada-Ruiz, E., Martínez-Cabrera, H. I. & Cevallos-Ferriz, S. R. S. Fossil woods from the late Campanian–early Maastrichtian Olmos Formation, Coahuila, Mexico. Rev. Palaeobot. Palynol. 45, 123–133 (2007).

    Article  Google Scholar 

  59. 59.

    Sainz-Resendiz, B. A., Estrada-Ruiz, E., Mateo-Cid, L. E. & Porras-Múzquiz, H. Primer registro de un estípite de Coryphoideae: Palmoxylon kikaapoa de la Formación Olmos del Cretácico Superior, Coahuila, México. Rev. Mex. Biodivers. 86, 872–881 (2015).

    Article  Google Scholar 

  60. 60.

    Pessagno, E. A. Upper Cretaceous Stratigraphy of the Western Gulf Coast Area of Mexico, Texas and Arkansas (Geological Society of America, 1969).

  61. 61.

    Martínez-Hernández, E., Almeida-Leñero, L., Reyes-Salas, M. & Betancourt-Aguilar, Y. Estudio palinológico para la determinación de ambientes en la cuenca Fuentes-Río Escondido (Cretácico Superior), región de Piedras Negras, Coahuila. Rev. del. Inst. de. Geol. ía. 4, 167–185 (1980).

    Google Scholar 

  62. 62.

    Flores-Espinoza, E. Stratigraphy and Sedimentology of the Upper Cretaceous terrigenous rocks and coal of the Sabinas-Monclova area, northern Mexico. Ph.D. Dissertation. University of Texas at Austin. (1989).

  63. 63.

    Imlay, R. W. Evolution of the Coahuila Peninsula, Mexico. Part IV. Geology of the Western part of the Sierra de Parras. GSA Bull. 47, 1091–1152 (1936).

    Article  Google Scholar 

  64. 64.

    Robeck, R. C., Pesquera, V. R., Ulloa, A. S. Geología y Depósitos De Carbón En La Región De Sabinas, Estado De Coahuila. 109. (XX Congreso Geológico Internacional. México, 1956).

  65. 65.

    Polhill, R. M. & Raven, P. H. (eds). Advances in Legume Systematics, Parts 1 and 2 (Royal Botanic Gardens, 1981).

  66. 66.

    Gunn, C. R. Fruits and Seeds of Genera in the Subfamily Mimosoideae (Fabaceae) Technical Bulletin No. 1681 (U.S. Department of Agriculture, 1984).

  67. 67.

    Gunn, C. R. Fruits and Seeds of Genera in the Subfamily Caesalpinioideae (Fabaceae) Technical Bulletin No. 1755 (U. S. Department of Agriculture, 1991).

  68. 68.

    Polhill, R. M. Phytochemical Dictionary of the Leguminosae (eds. Bisby, F. A. Buckingham, J. & Harborne J. B.) 35–48 (Chapman and Hall, 1994).

  69. 69.

    Kirkbride, J. H. Jr., Gunn, C. R. & Weitzman, A. L. Fruits and Seeds of Genera in the Subfamily Faboideae (Fabaceae) Technical Bulletin No. 1890 (U. S. Department of Agriculture. 2003).

  70. 70.

    Ulibarri, E. A. Los géneros de Caesalpinioideae (Leguminosae) presentes en Sudamérica. Darwiniana 46, 69–163 (2008).

    Google Scholar 

  71. 71.

    Tropicos. Botanical Information system at the Missouri Botanical Garden. (2020).

  72. 72.

    The Herbarium Catalogue. Royal Botanic Gardens, Kew. Published on the Internet. (2020).

  73. 73.

    Grether, R., Martínez-Bernal, A., Luckow M. & Zárate, S. Flora Del Valle De Tehuacán-Cuicatlán (eds. Novelo-Retana, A. G., Medina-Lemos, R., Ochoterena-Booth, H., Salazar-Chávez, G. A. & Alvarado-Cárdenas, L. O.) Vol. 44, 1–108 (Universidad Nacional Autónoma De México, 2006).

  74. 74.

    Gagnon, E., Bruneau, A., Hughes, C. E., de Queiroz, L. P. & Lewis, G. P. A new generic system for the pantropical Caesalpinia group (Leguminosae). PhytoKeys 71, 1–160 (2016).

    Article  Google Scholar 

  75. 75.

    Chen, Y. F. & Zhang, D. X. Bauhinia larsenii, a fossil legume from Guangxi, China Botanical. Bot. J. Linn. Soc. 147, 437–440 (2005).

    Article  Google Scholar 

  76. 76.

    Herendeen, P. S. Advances in Legume Systematics, Part 4 (eds. Herendeen, P. S. & Dilcher, D. L.) 85–160 (Royal Botanic Gardens, 1992).

  77. 77.

    Wang, Q., Manchester, S. R. & Dilcher, D. L. Fruits and foliage of pueraria (leguminosae, papilionoideae) from the neogene of eurasia and their biogeographic implications. Am. J. Bot. 97, 1982–1998 (2010).

    PubMed  Article  PubMed Central  Google Scholar 

  78. 78.

    Lee, S. K. Flora Reipublicae Popularis Sinicae. Vol. 41, 1–405 (Science Press, 1995).

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We are grateful for comments on an early version of this manuscript by Dr. Dori Contreras (Perot Museum of Nature and Science). Alexander Doweld (National Institute of Carpology) for his help in nomenclature. Thank you very much for the support to the technicians at the Herbarium of the Instituto de Biología, UNAM. This work was improved by two anonymous reviewers, which we appreciate to much. This research has been funded by CONACyT (240241) grants to E.E.R.

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E.E.R. is responsible for and coordinated the project; N.K.C.G., E.E.R., and H.P.M. collected the fossil; N.K.C.G., H.P.M., H.I.M.C., and E.E.R. wrote the manuscript and contributed to discussions. N.K.C.G., H.I.M.C., and E.E.R. made all the figures. All authors have read, commented on, and approved the final paper.

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Correspondence to Emilio Estrada-Ruiz.

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Centeno-González, N.K., Martínez-Cabrera, H.I., Porras-Múzquiz, H. et al. Late Campanian fossil of a legume fruit supports Mexico as a center of Fabaceae radiation. Commun Biol 4, 41 (2021).

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