Abstract
Wasabi, horseradish and mustard are popular pungent crops in which the characteristic bioactive hydrolysis of specialized glucosinolates (GSLs) occurs. Although the metabolic pathways of GSLs are well elucidated, how plants have evolved convergent mechanisms to accumulate identical GSL components remains largely unknown. In this study, we discovered that sinigrin is predominantly synthesized in wasabi, horseradish and mustard in Brassicaceae. We de novo assembled the transcriptomes of the three species, revealing the expression patterns of gene clusters associated with chain elongation, side chain modification and transport. Our analysis further revealed that several gene clusters were convergently selected during evolution, exhibiting convergent shifts in amino acid preferences in mustard, wasabi and horseradish. Collectively, our findings provide insights into how unrelated crop species evolve the capacity for sinigrin super-accumulation and thus promise a potent strategy for engineering metabolic pathways at multiple checkpoints to fortify bioactive compounds for condiment or pharmaceutical purposes.
Similar content being viewed by others
Introduction
Plants synthesize a multitude of compounds that help them adapt to their ecological niches, but also increase their popularity as a source of food for humans1. Glucosinolates (GSLs) are specialized metabolites in the family Brassicaceae2,3 that act as protectants against generalists4,5, and can mediate the coevolutionary association between herbivores and plants. The bioactive products of GSL hydrolysis, typically isothiocyanates and nitriles6,7, are associated with improved health benefits7. Well-known allyl-isothiocyanate or benzyl-isothiocyanate (ITC) is a characteristic determinant of the pungent flavor associated with wasabi. The products of GSL hydrolysis, especially sinigrin-derived ITCs, are major determinants of the pungent aroma of wasabi (Wasabi japonica) roots, horseradish (Armoracia rusticana) roots and mustard (Brassica juncea) seeds8,9,10, which release ITC when grated11. Moreover, the breakdown of sinigrin contributes to the characteristic taste of mustard12,13. Progress has been made over the last two decades with regard to creating a model ecological system for identifying causal genes underlying natural variation, and this model features the biosynthesis of GSLs and both storage and transport pathways3. Understanding the evolutionary and genetic bases of such metabolic diversity and specificity, could provide crucial clues for chemical ecology and yield useful tools for crop breeding. Such an understanding could also create opportunities for producing and engineering plant-derived chemicals for the biofortification of certain pharmaceutical applications.
Convergent evolution is the appearance of similar phenotypes in distinct evolutionary lineages14,15, and is frequently observed for specialized metabolites in plants1,16,17. A particularly intriguing goal in evolutionary biology is to understand the genetic mechanisms, that result in different species exhibiting the same levels of variation in specialized metabolites. A large number of genetic and biochemical mechanisms could give rise to convergent evolution. Moreover, the convergent evolution of specialized metabolism in plants is surprisingly common and predominantly involves the same product from the same substrate, but with unrelated enzymes and genes or the same product from different substrates1.
The synthesis of GSLs is emerging as a unique model with which to investigate nonlinear selection and a blend of convergent, divergent, and parallel evolution that shapes natural variation7,18. However, we do not yet fully understand how the selectivity and evolution of GSL transport may underlie natural variation within and between species. Early studies showed that de novo biosynthesis does not occur in seeds19, and it is generally assumed that GSLs are synthesized along the vasculature in leaves and subsequently transported from the leaves to the seeds20. Recent studies demonstrated that two Arabidopsis transporters, AtNPF2.10 (AtGTR1) and AtNPF2.11 (AtGTR2), are responsible for transporting GSLs, particularly long-chain aliphatic GSLs21,22,23. In roots, GTR-mediated import is essential for the retention of GSLs in the gtr1gtr2 mutant, and GSLs are moved from root to shoot24. In contrast, short-chain aliphatic GSLs have been shown to be mainly produced in the leaves and then transported to the roots24. Furthermore, GTR1, GTR2, and GTR3 (AtNPF2.9) all contribute to distributing indole GSLs between roots and shoots25.
In general, a large amount of diversity exists in both the amount and profile of GSLs in Brassicaceae crops26, thus resulting in a range of different flavors. The diverse components that make up GSLs are thought to need different transporters in Brassicaceae crops and the model plant Arabidopsis. However, we have yet to determine how plant lineages from families that are distantly related to Brassicaceae have evolved mechanisms to accumulate GSLs. In the present study, we used comparative transcriptome analysis to dissect the major causal loci and gene clusters underlying the convergent evolution of GSL accumulation.
Results
Comparative analyses of GSLs in wasabi, horseradish, and mustard
We constructed a phylogenetic tree for wasabi, horseradish and mustard species with several other sequenced species using one-to-one orthologs in the Brassicaceae family. The wasabi, horseradish, and mustard species all belonged to different lineages within the family (Fig. 1a). According to the definition of convergent evolution, the accumulation of sinigrin in these three species is considered a result of convergent evolution, because similar phenotypes are evident in distinct evolutionary lineages within the same family15. We determined GSL components in the leaves, roots, and seeds using high-performance liquid chromatography (HPLC). The content of aliphatic GSLs was much more intensively enriched than the content of indolic GSLs in the leaves, roots, and seeds of these three species (Supplementary Fig. 1 and Supplementary Data 1). 3C-aliphatic-sinigrin is a predominant component in the leaves of all of the species (Fig. 1b and Supplementary Data 2). 3C-aliphatic-sinigrin accumulates far more extensively in the harvestable roots of wasabi and horseradish than in mustard (Fig. 1b and Supplementary Data 2). We observed that wasabi and mustard seeds mainly accumulate sinigrin, while horseradish seeds accumulate a greater proportion of gluconapin (Fig. 1b and Supplementary Data 2). GSLs have been shown to be synthesized in various tissues, including the root, leaf, stem, and silique wall, and then translocated from source to sink20,21,25. These results suggested the convergent accumulation of sinigrin in these three species.
The de novo assembly of transcriptomes for wasabi, horseradish and mustard
We de novo assembled the transcriptomes of W. japonica, A. rusticana, and B. juncea. Twenty-seven samples of leaves, roots, and seeds were collected in triplicate for Illumina sequencing. Following data assessment, quality control, filtration, and assembly, we obtained an average of 5.46 Gb of clean data for each sample. This generated 53,660, 61,283, and 69,279 unigenes for these three species, respectively (Table 1, Supplementary Data 3–5, and Supplementary Fig. 2). We estimated the transcriptome assemblies using BUSCO, in which over 50% of the gene models were annotated for the three species (Supplementary Data 6). In total, 64,774 common transcripts were shared across the three transcriptomes, and specific transcripts were identified in all three species (Fig. 2a). The biological replicates used for transcriptome sequencing of the leaves, roots, and seeds of these three species were validated using principal component analysis. These analyses indicated that the expression patterns showed high levels of similarity between biological replicates (Fig. 2b).
We identified genes related to the biosynthesis, transport, and breakdown of GSLs based on the metabolic pathways of GSLs from the three species and their orthologs from Arabidopsis (Supplementary Data 7). Next, we investigated the transcriptional expression of genes involved in the metabolic pathways of GSLs, including side chain modifications, transcription factors, and transporters within the NPF family (Fig. 2c–f and Supplementary Fig. 3 and Supplementary Data 8). Analysis indicated that some identical functional genes (IGMT1, FMOGS-OX1, FMOGS-OX4, and CYP81F1) showed similar transcriptional patterns in the leaves of all species but also revealed that several diverse genes (GSL-OH, FMOGS-OX3, and CYP81F4) were expressed at similar levels in the leaves of the two species (Fig. 2d–f). However, we found that some genes involved in the modification of GSL side chains were expressed at higher levels in roots, suggesting that GSLs might also be synthesized in the roots (Fig. 2d). Previous research also demonstrated that GSLs, particularly indole GSL, can be synthesized in both the leaves and the roots27. We confirmed that AOP2 was expressed at significantly higher levels in leaves than in seeds and roots (Supplementary Fig. 4), in which this gene plays a key role in the biosynthesis of glucosinolates that then convert glucoiberin to sinigrin. With regard to the transcriptional regulation of GSL biosynthesis, we found that the transcription factor Dof1.1 exhibited the highest expression levels in all three species and that the transcription factors MYB28 and IQD exhibited higher expression levels in two of the species (Fig. 2e). The higher expression levels of genes in the NPF family in leaves and roots of the three species suggested that these genes are potential transporters of GSLs from sink to source (Fig. 2f).
Phylogenetic evolutionary analysis of wasabi, horseradish, and mustard
We then collected single-copy genes from the A subgenomes of B. juncea and B. napus and from A. thaliana, A. rusticana, B. oleracea, B. rapa, and W. japonica in Brassicaceae. Using these single-copy-gene orthologs, we constructed a phylogenetic tree showing independent evolutionary lineages for B. juncea, A. rusticana, and W. japonica (Fig. 3a). Sinigrin is specifically enriched in several species of Brassicaceae, including B. nigra, B. juncea, and B. carinata, but not in B. rapa26 and several species in other Brassicaceae clusters, including W. japonica and A. rusticana10. We calculated the Ka and Ka/Ks (nonsynonymous/synonymous) values of all single-copy genes and GSL-related genes in these seven species. The Ka values of B. juncea, A. rusticana, W. japonica, and A. thaliana were much higher than those of B. rapa, B. oleracea, and B. napus (Fig. 3b and Supplementary Fig. 5), suggesting parallel selection within these species. We also used positively selected gene (PSG) analysis to identify candidate selected genes in A. rusticana, B. juncea and W. japonica. A. rusticana, B. juncea, and W. japonica were used as the foreground branches while B. napus, B. oleracea, B. rapa, and A. thaliana were used as background branches in the PSG analysis. This analysis showed that 354 genes were under positive selection in B. juncea, A. rusticana, and W. japonica (Supplementary Data 9).
Convergent evolution analysis in wasabi, horseradish, and mustard
Next, we used the PCOC package, which allows for the accurate detection of convergent shifts in amino acid preferences28, to identify nine genes exhibiting convergent evolution in B. juncea, A. rusticana, and W. japonica, using B. rapa as a control. These genes are involved in the biosynthesis (CYP79A1, CYP83A1, GSTF11, SUR1, and UTG74B1), transcriptional regulation (Dof1.1 and IQD) and breakdown (NIT1) of GSLs (Fig. 4 and Supplementary Data 10). The amino acids encoded by these genes displayed convergent shifts in B. juncea, A. rusticana, W. japonica, and A. thaliana but showed divergent variations in B. rapa, B. oleracea, and B. napus (Fig. 4). For example, the amino acids (Q233L and K364N) in the SUR1 gene are involved in the core structural formation of GSLs from S-alkyl-thiohydroximate to thiohydroximate and showed convergent substitutions in species known to accumulate large amounts of sinigrin (B. juncea, A. rusticana, and W. japonica) compared to other species (B. rapa, B. oleracea, and B. napus) in the phylogenetic tree (Fig. 4). In addition, several other amino acids (74th, 122nd, and 185th) showed convergent shifts in B. juncea, A. rusticana, and W. japonica compared to B. rapa, B. oleracea, and B. napus in the phylogenetic tree (Fig. 4). These results suggested that the convergent evolution of genes associated with the metabolism of GSLs may contribute to the convergent enrichment of sinigrin in B. juncea, A. rusticana, and W. japonica.
Discussion
The process of convergent evolution, in which distinct lineages independently evolve similar traits, has fascinated evolutionary biologists for centuries29, predominantly because convergent evolution is generally thought to represent a visible manifestation of the power of selection. The plant and animal kingdoms evolve similar traits under selection in order to adapt to different ecological environments. However, most of the existing research on convergent or parallel evolution is focused on morphological variations, and very little is known about metabolic pathways and the release of bioactive metabolites in Brassicaceae. Previous studies on the evolution of Brassicaceae indicated the prevalence of parallel or convergent evolution of several traits over time across the entire family30. Indeed, recent publications have reported that several specific traits, including tetraterpene biosynthesis31, leaf head formation32, and flowering time33, have undergone parallel or convergent domestication or selection.
With the development of high-throughput sequencing technologies, numerous research studies have begun to investigate the specific genetic mechanisms, that underlie convergent traits and to test whether and under which conditions phenotypic convergence is associated with convergence at the genetic level33. Prior to the availability of genomic sequences, the transcriptomes of orphan species were of benefit in the identification of genes involved in GSL metabolism. Plants in the Brassicaceae family are now becoming an attractive model for studying the evolution of bioactive compounds. This is because these plants contain an abundance of GSLs and because their biosynthetic pathways are well documented. In the present study, we used de novo transcriptome assembly and PSG and PCOC analyses to identify the genetic mechanisms associated with the convergent evolution of sinigrin super-accumulation in mustard, wasabi, and horseradish. We found that the evolution of aliphatic GSLs is associated with multiple genes in a diverse array of pathways, and that the natural variations in alkenyl GSLs are caused by parallel nonfunctional alleles in the conserved AOP2 locus. As exemplified by the GS-OH locus, the three different lineages of Brassicaceae most likely evolved the same components of GSLs by coopting different enzymes. Furthermore, it was found that the Elong locus has been subject to a complex combination of divergent and convergent evolution18. In this study, we found that convergent shifts of amino acids were largely imposed on genes involved in the formation of core structures and transcriptional regulation of GSLs. These observations imply that selection on these genes in core pathways leads to convergent evolution for the super-accumulation of sinigrin-ITCs in three distantly related species of Brassicaceae. This finding provides insights into how to select convergent GSL biosynthetic targets via metabolic engineering in order to enhance the synthetic capacity of leaf sources and improve certain traits in Brassicaceae. This is important because sinigrin is present in a range of vegetables within the Brassicaceae family, including broccoli, Brussels sprouts, black mustard seeds, and wasabi.
Metabolic gene clusters that are associated with specialized metabolism in plants represent the genomic signatures for metabolic evolution, define the specific features of plants and provide us with options for exploiting such plants with regard to their synthetic biological applications34. By investigating gene clusters associated with GSL diversity in Brassicaceae35, it is possible to enhance knowledge of taxonomy and identify plants with greater agricultural potential and abiotic stress tolerance36. Further studies are now needed to investigate whether these transporter genes are clustered in genomic regions with coregulatory mechanisms. In the present study, we used the comparative transcriptomes of three species of Brassicaceae to elucidate the evolutionary trajectories of crucial bioactive compounds formed from GSLs. Common selection on genes involved in biosynthetic and transport pathways plays a key role in the super-accumulation of sinigrin. Our findings will further advance our understanding of convergent evolution in relation to how plants super-accumulate specific bioactive compounds for improving environmental fitness. Moreover, our work opens up the possibility of developing toolkits to engineer metabolic pathways, so that we can use synthetic biology to improve the synthesis of bioactive compounds for condimental or pharmaceutical purposes.
Methods
Plant materials and GSL component assays
Three Brassicaceae species were used to study the convergent evolution of wasabi quality: wasabi (Wasabi japonica), horseradish (Armoracia rusticana), and mustard (Brassica juncea). We collected leaves, roots and seeds from all three species for transcriptome analysis. The leaf and root samples were collected when the root organs had formed, and the seeds were collected when mature in the population. GSL profiles were analyzed by HPLC in accordance with standard protocols37.
De novo transcriptome assemblies of B. juncea, A. rusticana, and W. japonica
Total RNA was extracted from the leaves, roots, and seeds of the three species using TRIzol Reagent (Life Technologies, California, USA). We then constructed a cDNA library for each sample according to the instruction manual (Illumina, USA) and sequenced PE150 fragments using an Illumina HiSeq™ 2500 sequencing platform (Illumina, USA) with a standard pipeline. Quality control measures were then applied, and the Trinity pipeline was used to create de novo assemblies from the clean data38 with the following parameter settings: min_glue = 3, V = 10, edge-thr = 0.05, min_kmer_cov = 3, path_reinforcement_distance = 85, group_pairs_distance = 250. All other parameters were set as the default. Next, we removed any redundant fragments using TGICL (TGI Clustering tools) and the Phrap assembler39. The following parameters were then used to ensure that the assemblies were of high quality: a minimum identity of 95%, a minimum of 35 overlapping bases, a minimum of 35 scores, and a maximum of 25 unmatched overhanging bases at sequence ends.
Calculation of Ka and Ks
Based on a phylogenetic tree of seven plant species (A. thaliana, A. rusticana, B. juncea, B. napus, B. oleracea, B. rapa, and W. japonica) and one-to-one orthologous genes, we estimated the evolutionary rates (Ka and Ks) of each branch using the CodeML tool in the PAML package (version 4.9 h) and the free-ratio “branch” model40, which allows distinct evolutionary rates for the branches.
Positive selection analysis
Based on the phylogenetic tree created for the three species of Brassicaceae, we used the branch-site model incorporated in the PAML package to detect positively selected genes (PSGs). The null model used in the branch-site test assumed that the Ka/Ks values for all codons on all branches were ≤1, whereas the alternative model assumed that the foreground branch included codons evolving with a Ka/Ks >1. A maximum likelihood ratio test (LRT) was used to compare the two models. The p value was calculated according to the chi-square distribution with 1 degree of freedom (df = 1). Then, the p values were adjusted for multiple testing using the false discovery rate (FDR) method. Genes were identified as being positively selected when the FDR < 0.05. Furthermore, we required that at least one amino acid site possess a high probability of being positively selected (Bayes probability >95%). If none of the amino acids passed this cutoff in the PSG analysis, then these genes were identified as false positives and excluded.
Convergent evolution analysis
One-to-one orthologs were identified in seven Brassicaceae species (A. thaliana, A. rusticana, B. juncea, B. napus, B. oleracea, B. rapa, and W. japonica). To detect the convergent evolution of genes among A. rusticana, B. juncea, and W. japonica, we used the Profile Change with One Change (PCOC) package with a posterior probability threshold of 0.95 to detect convergent amino acids for each gene28. Because B. rapa does not accumulate sinigrin26, we further required at least one candidate convergent amino acid for A. rusticana, B. juncea, and W. japonica that was different from that in B. rapa.
Statistics and reproducibility
All RNA-sequencing data were from three independent biological replications. The GSL components and gene expression analysis were performed with three independent biological replications in this study.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
All the transcriptome data, including those for unigenes and differentially expressed genes, generated in the present study have been deposited in NCBI (https://www.ncbi.nlm.nih.gov/geo/) under accession number PRJNA670607, and in CNSA (https://db.cngb.org/cnsa/) under accession number CNP0001119.
References
Pichersky, E. & Lewinsohn, E. Convergent evolution in plant specialized metabolism. Annu. Rev. Plant Biol. 62, 549–566 (2011).
Agerbirk, N. & Olsen, C. E. Glucosinolate structures in evolution. Phytochemistry 96, 466–467 (2013).
Halkier, B. A. General introduction to gslucosinolates. Adv. Bot. Res. 80, 1–14 (2016).
Jeschke, V., Gershenzon, J. & Vassao, D. G. Insect detoxification of glucosinolates and their hydrolysis products. Adv. Bot. Res. 80, 199–245 (2016).
Pastorczyk, M. & Bednarek, P. The function of glucosinolates and related metabolites in plant innate immunity. Adv. Bot. Res. 80, 171–198 (2016).
Halkier, B. A. & Gershenzon, J. Biology and biochemistry of glucosinolates. Annu. Rev. Plant Biol. 57, 303–333 (2006).
Traka, M. H. Health benefits of glucosinolates. Adv. Bot. Res. 80, 247–279 (2016).
Yu, E. Y., Pickering, I. J., George, G. N. & Prince, R. C. In situ observation of the generation of isothiocyanates from sinigrin in horseradish and wasabi. BBA 1527, 156–160 (2001).
Popova, I. E. & Morra, M. J. Simultaneous quantification of sinigrin, sinalbin, and anionic glucosinolate hydrolysis products in Brassica juncea and Sinapis alba seed extracts using ion chromatography. J. Agr. Food Chem. 62, 10687–10693 (2014).
Terada, Y., Masuda, H. & Watanabe, T. Structure-activity relationship study on isothiocyanates: comparison of TRPA1-activating ability between allyl Isothiocyanate and specific flavor components of Wasabi, Horseradish, and White Mustard. J. Nat. Prod. 78, 1937–1941 (2015).
Fahey, J. W., Zalcmann, A. T. & Talalay, P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56, 5–51 (2001).
Rochfort, S. J., Trenerry, V. C., Imsic, M., Panozzo, J. & Jones, R. Class targeted metabolomics: ESI ion trap screening methods for glucosinolates based on MSn fragmentation. Phytochemistry 69, 1671–1679 (2008).
Cools, K. & Terry, L. A. The effect of processing on the glucosinolate profile in mustard seed. Food Chem. 252, 343–348 (2018).
Arendt, J. & Reznick, D. Convergence and parallelism reconsidered: what have we learned about the genetics of adaptation? Trends Ecol. Evol. 23, 26–32 (2008).
Washburn, J. D., Bird, K. A., Conant, G. C. & Pires, J. C. Convergent evolution and the origin of complex phenotypes in the age of systems biology. Int. J. Plant Sci. 177, 305–318 (2016).
Zhou, Y. et al. Convergence and divergence of bitterness biosynthesis and regulation in Cucurbitaceae. Nat. Plants 2, 16183 (2016).
Denoeud, F. et al. The coffee genome provides insight into the convergent evolution of caffeine biosynthesis. Science 345, 1181–1184 (2014).
Kliebenstein, D. J. & Cacho, N. I. Nonlinear selection and a blend of convergent, divergent and parallel evolution shapes natural variation in glucosinolates. Adv. Bot. Res. 80, 31–55 (2016).
Magrath, R. & Mithen, R. Maternal effects on the expression of individual aliphatic glucosinolates in seeds and seedlings of Brassica napus. Plant Breed. 111, 249–252 (1993).
Chen, S. X., Petersen, B. L., Olsen, C. E., Schulz, A. & Halkier, B. A. Long-distance phloem transport of glucosinolates in Arabidopsis. Plant Physiol. 127, 194–201 (2001).
Nour-Eldin, H. H. et al. NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds. Nature 488, 531–534 (2012).
Nour-Eldin, H. H. et al. Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. Nat. Biotechnol. 35, 377–382 (2017).
Madsen, S. R., Olsen, C. E., Nour-Eldin, H. H. & Halkier, B. A. Elucidating the role of transport processes in leaf glucosinolate distribution. Plant Physiol. 166, 1450–1462 (2014).
Andersen, T. G. et al. Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis. Plant Cell 25, 3133–3145 (2013).
Jorgensen, M. E. et al. Origin and evolution of transporter substrate specificity within the NPF family. Elife 6, e19466s (2017).
Ishida, M., Hara, M., Fukino, N., Kakizaki, T. & Morimitsu, Y. Glucosinolate metabolism, functionality and breeding for the improvement of Brassicaceae vegetables. Breed. Sci. 64, 48–59 (2014).
Jorgensen, M. E., Nour-Eldin, H. H. & Halkier, B. A. Transport of defense compounds from source to sink: lessons learned from glucosinolates. Trends Plant Sci. 20, 508–514 (2015).
Rey, C., Gueguen, L., Semon, M. & Boussau, B. Accurate detection of convergent amino-acid evolution with PCOC. Mol. Biol. Evol. 35, 2296–2306 (2018).
Darwin, C. On the Origin of Species (John Murray, London, 1859).
Huang, C. H. et al. Resolution of Brassicaceae phylogeny using nuclear genes uncovers nested radiations and supports convergent morphological evolution. Mol. Biol. Evol. 33, 394–412 (2016).
Huang, A. C. C. et al. Unearthing a sesterterpene biosynthetic repertoire in the Brassicaceae through genome mining reveals convergent evolution. Proc. Natl Acad. Sci. USA 114, E6005–E6014 (2017).
Cheng, F. et al. Subgenome parallel selection is associated with morphotype diversification and convergent crop domestication in Brassica rapa and Brassica oleracea. Nat. Genet. 48, 1218–1224 (2016).
Gould, B. A. & Stinchcombe, J. R. Population genomic scans suggest novel genes underlie convergent flowering time evolution in the introduced range of Arabidopsis thaliana. Mol. Ecol. 26, 92–106 (2017).
Nutzmann, H. W. & Osbourn, A. Gene clustering in plant specialized metabolism. Curr. Opin. Biotechnol. 26, 91–99 (2014).
Kliebenstein, D. J., Lambrix, V. M., Reichelt, M., Gershenzon, J. & Mitchell-Olds, T. Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in arabidopsis. Plant Cell 13, 681–693 (2001).
Essoh, A. P. et al. Exploring glucosinolates diversity in Brassicaceae: a genomic and chemical assessment for deciphering abiotic stress tolerance. Plant Physiol. Biochem. 150, 151–161 (2020).
Miao, H. Y. et al. Glucose signalling positively regulates aliphatic glucosinolate biosynthesis. J. Exp. Bot. 64, 1097–1109 (2013).
Grabherr, M. G. et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29, 644–U130 (2011).
Pertea, G. et al. TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. Bioinformatics 19, 651–652 (2003).
Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Acknowledgements
The authors thank Dr. H. Miao for GSL component analyses. This work was supported by the National Natural Science Foundation of China (32030092, 31872095), the National Natural Science Foundation of Zhejiang Province (LZ20C150002), and the Cheung Kong Youth Scholar Chair Professor of the Ministry of Education.
Author information
Authors and Affiliations
Contributions
J.Y. and M.Z. designed the research. Z.L. and G.Q. analyzed glucosinolate components. J.L. and J.Y. performed comparative genomic and evolutionary analyses. P.S., J.H., and Z.H. collected some data from the experiments. J.Y. wrote the paper. M.Z. revised the paper.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Yang, J., Li, Z., Lian, J. et al. Brassicaceae transcriptomes reveal convergent evolution of super-accumulation of sinigrin. Commun Biol 3, 779 (2020). https://doi.org/10.1038/s42003-020-01523-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s42003-020-01523-x
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.