Fig. 3: Chip-based TIRF and dSTORM imaging combined with FIB-SEM of LSECs, sample 1. | Communications Biology

Fig. 3: Chip-based TIRF and dSTORM imaging combined with FIB-SEM of LSECs, sample 1.

From: Photonic-chip assisted correlative light and electron microscopy

Fig. 3

a Chip-based TIRF and dSTORM overlay of a LSEC including a correlation to the corresponding FIB-SEM datastack. b Chip-based TIRF showing AF488-FSA (in red) internalized in vesicles upon 2 h incubation with the cells. c 3D reconstruction of lysosomes based on the FIB-SEM datastack and the x/y re-sliced imaging plane (manually segmented in light blue). d chip-based dSTORM of the cell surface displaying fenestrations (in encircled areas).

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