(a) 40 classes of 70S ribosome are produced by ISAC. (b) 45 classes are produced by P-ISAC. (c) 37 classes of beta-galactosidase are produced by ISAC. (d) 41 classes of beta-galactosidase are produced by P-ISAC. (e) and (f) show the initial models calculated from (c) and (d), respectively, where the calculation was performed with PRIME. The initial model from ISAC is in green and the one from P-ISAC is in blue. (g) shows the comparison of the initial models to the golden model. The green FSC curve shows (e) to the golden model while the blue FSC curve shows (f) to the golden model.