Fig. 3: OFO enhances ethanol-induced intestinal barrier dysfunction. | Communications Biology

Fig. 3: OFO enhances ethanol-induced intestinal barrier dysfunction.

From: Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice

Fig. 3

a Immunofluorescent staining of intestinal TJ proteins, including ZO-1, claudin-4, occludin, and claudin-2, in jejunum tissue (scale bar, 25 μm). b Immunoblot analysis of intestinal TJ proteins. c Plasma LPS levels (n = 5–6). d The hepatic expressions of gram-negative bacteria 16S rRNA (n = 7). e Fecal LPS contents (n = 7). f Immunofluorescent staining of TNF-α (green) and F4/80 (red) in the sections of jejunum tissue (scale bar, 25 μm). Data were expressed as mean ± SD. Labeled means without a common letter differ within the column (p < 0.05). CO corn oil, OFO oxidized fish oil, FO fish oil.

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