Fig. 1 | Communications Biology

Fig. 1

From: High-efficiency genomic editing in Epstein-Barr virus-transformed lymphoblastoid B cells using a single-stranded donor oligonucleotide strategy

Fig. 1

Overview of the LCL editing protocol. We start by transfecting 4 million cells with the plasmid expressing Cas9 and the guide RNA, as well as a green fluorescent protein (GFP) to allow us to sort the transfected cells. The numbers of cells at each stage are shown. Substantial cell death occurs following transfection, but the cell cloning steps were relatively efficient, and the rate of recovery of edited clones was high

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