Iron (Fe) bioavailability limits phytoplankton growth in vast ocean regions. Iron-rich dust uplifted from deserts is transported in the atmosphere and deposited on the ocean surface. However, this dust is a poor source of iron for most phytoplankton since dust-bound Fe is poorly soluble in seawater and dust rapidly sinks out of the photic zone. An exception is Trichodesmium, a globally important, N2 fixing, colony forming, cyanobacterium, which efficiently captures and shuffles dust to its colony core. Trichodesmium and bacteria that reside within its colonies carry out diverse metabolic interactions. Here we show evidence for mutualistic interactions between Trichodesmium and associated bacteria for utilization of iron from dust, where bacteria promote dust dissolution by producing Fe-complexing molecules (siderophores) and Trichodesmium provides dust and optimal physical settings for dissolution and uptake. Our results demonstrate how intricate relationships between producers and consumers can influence productivity in the nutrient starved open ocean.
In large parts of the ocean, supply of the nutrients iron (Fe), phosphorous (P), and nitrogen (N) limit phytoplankton growth1. Some phytoplankton supply their N-demands by fixing the inert gaseous nitrogen (N2) into biologically accessible nitrogen and further fuel the ocean primary productivity by releasing excess fixed-nitrogen2,3. The cyanobacterium Trichodesmium spp., an important ecosystem player in oligotrophic ocean regions, contributes to ~50% of marine N2-fixation and forms extensive surface blooms visible even from space4,5 (Fig. 1a). Large fluxes of nutrients, organic molecules, and toxins released from Trichodesmium blooms have strong impact on both chemical and biological components of marine ecosystems3,6,7 (Fig. 1a). Trichodesmium appears typically as free filaments (trichomes) in the water-column or as colonies composed of tens to hundreds of individual trichomes8,9. Colonies of Trichodesmium host many associated bacteria which are distinct from free-living bacteria in seawater10,11,12. Trichodesmium and its associated bacteria exchange nutrients and organic molecules between them and act together to optimize the growth of the whole consortium13,14.
Atmospheric dust is considered an important source of iron to Fe-poor ocean regions, but the rapid sinking of dust from the ocean surface and the low solubility of iron from dust (dust-Fe) restricts its utilization by phytoplankton15,16,17. Buoyant Trichodesmium colonies overcome these constraints by efficient trapping of dust particles deposited at the ocean surface and subsequent shuffling of dust to the colony center, where it is protected from loss18,19 (Fig. 1b). In addition to dust capturing, Trichodesmium colonies were shown to chemically modify dust and increase dust-Fe solubility and bioavailability19,20. The two most common mechanisms microorganisms apply for dissolving mineral-Fe are reductive dissolution and siderophore promoted dissolution, both of which were suggested to play a role in Trichodesmium-dust interactions19,20. In reductive dissolution, conversion of mineral Fe(III) to soluble Fe(II) facilitates dissolution21. In siderophore promoted dissolution, Fe-specific ligands react with Fe(III) at the mineral surface and then the Fe-siderophore complexes return to solution21,22.
A large group of siderophores, produced by bacteria, fungi, and cyanobacteria, are involved in active dissolution of Fe-minerals in many terrestrial and aquatic environments23,24. In the ocean, siderophores from the ferrioxamine group are frequently detected in surface waters and hence are considered important for the marine Fe-cycle24,25. Although Trichodesmium captures and shuffles dust to its colony core (Fig. 1b), it does not possess known pathways for siderophore synthesis and hence in isolation cannot utilize siderophore promoted dissolution for dissolving dust-bound Fe26. However, some of the bacteria residing within natural Trichodesmium colonies have the ability to produce siderophores26. We therefore hypothesize that bacteria associated with Trichodesmium colonies increase solubility of dust-bound Fe by releasing siderophores (Fig. 1c-I) that dissolve iron from dust trapped within the colony center (Fig. 1c-II). The siderophore-mediated dust dissolution would be beneficial for Trichodesmium if it can utilize the Fe that is complexed by siderophores (Fig. 1c-III). In this scenario, the bacterial strategy of Fe dissolution from dust by siderophores is favorable for Trichodesmium and thus of mutual advantage for the consortium.
In this contribution, we explored the role of biotic interactions in actively mining dust-bound iron within Trichodesmium colonies. Firstly, we examined the occurance of siderophores in natural Trichodesmium blooms from the coastal Arabian Sea and the Gulf of Aqaba at the northern end of the Red Sea. We detected siderophores in all Trichodesmium blooms and observed active siderophore production in response to dust addition. Then, using radiolabeled 55Fe-oxyhydroxide (55ferrihydrite) and natural colonies from the Gulf of Aqaba, we examined the effect of siderophores on mineral-Fe dissolution and uptake by both members of the Trichodesmium consortium. We found that addition of siderophores increased 55ferrihydrite dissolution and iron uptake in natural colonies. The siderophore promoted 55ferrihydrite dissolution benefited both Trichodesmium and its associated bacteria. Lastly, using β-imaging we show that iron uptake from 55ferrihydrite occurred mainly in the colony center, due to reduced diffusive losses of siderophores and their Fe-complexes. Thus, the colonies form a symbiotic community where Trichodesmium is a primary producer, from which the colony is constructed, thereby creating a shielded microenvironment, in which bacteria make the limiting nutrient iron bioavailable for the host.
Siderophore production by isolates of associated bacteria
First, we confirmed that associated bacteria from natural Trichodesmium colonies can produce siderophores in culture. We repeatedly plated individual Trichodesmium colonies from the Gulf of Aqaba on nutrient rich marine agar medium and isolated 23 bacterial strains. When grown in Fe-limited liquid media, the majority of these isolates (~75%) were screened as Chrome Azurol-S assay positive, which is indicative of siderophore production (Supplementary Fig. 1). These findings add up to previous genetic and physiological reports confirming the wide occurrence of this trait within Trichodesmium’s consortium26,27. In contrast, we analyzed a supernatant of cultured Fe-limited Trichodesmium erythraeum (strain IMS101) using high-performance liquid chromatography electrospray ionization – mass spectrometry (HPLC-ESI-MS)28 and observed no known siderophores.
Occurrence of ferrioxamine siderophores in Trichodesmium blooms
Next, we studied the occurrence of the ubiquitous marine siderophores, ferrioxamines B, G, and E24,25,29 in Trichodesmium blooms from the Arabian Sea and Gulf of Aqaba (Supplementary Fig. 2), employing high-resolution HPLC-ESI-MS24,28. These surface blooms contained buoyant healthy colonies at moderate to high densities of 2–27 × 105 trichomes L−1 (Fig. 2). Colony microbes were found at concentrations of 6–41 × 103 bacteria per trichome (Supplementary Table 1), in accord with reported values from Trichodesmium blooms worldwide10,30. This density amounted to 7–15 × 109 bacteria per liter (Fig. 2), which is 10–20 times higher than their density in seawater. Ferrioxamine concentrations were lower in the Arabian Sea bloom than in the Gulf of Aqaba bloom (2 pM and 45 pM, respectively, Fig. 2), and are in the range observed in surface seawater24,25. Normalizing in situ ferrioxamine concentrations to bacterial biomass, measured values were 0.2–3 × 10−21 mol per bacteria, comparable or slightly lower than ratios reported for open water in the Atlantic Ocean24.
Siderophore production by Trichodesmium consortium in the presence of dust
The presence of siderophores in Trichodesmium surface blooms implies that in an event of dust deposition dust-bound Fe may be dissolved by siderophores and made available to both members of the consortium. Given the high Fe requirements of Trichodesmium and the low solubility of Fe in dust, it is possible that the Trichodesmium consortium is further tuned for active mining of dust-bound Fe and may produce siderophores in respond to dust inputs. Hence, we conducted short-term incubations with natural Trichodesmium and probed for stimulation of ferrioxamine production by added dust. Colonies from natural blooms were washed and suspended in filtered seawater at ambient densities, while monitoring for changes in Trichodesmium biomass, which did not change over the course of the incubation. Bacterial cell counts, on the other hand, increased in all incubations (Fig. 3). The added local desert dust contained ~10 mg Fe g−1 dust, with a labile fraction of ~2 mg Fe g−1 dust (acetic acid extraction31), and it likely released additional dissolved nutrients and trace metals to the incubation water32.
As a result of Trichodesmium transfer and wash, ferrioxamine concentrations were low or below detection limit (bdl) in the initial time point of the incubations (Fig. 3, To bars). Ferrioxamine were actively produced during all incubations with final concentrations ranging from 7 pM to 1 nM, and ferrioxamine types differed among sites (Fig. 3, Tfinal bars). Dust strongly enhanced ferrioxamine production in three of the four incubations, where total ferrioxamine concentrations in the presence of dust increased by up to 12-fold compared to the incubations without dust (Fig. 3, Tfinal and Tfinal+Dust bars). Ferrioxamines were not detected in abiotic control incubations with dust only. Dust had no effect on Trichodesmium biomass, but it strongly enhanced bacterial growth in two of the incubations (Fig. 3). We can rule out supply of bacteria from the dust, since it was sterilized by UV irradiation prior to the experiments. Our findings on enhanced siderophore production in response to dust therefore imply that the Trichodesmium consortium has a mechanism to increase the supply of iron from atmospheric dust deposited in the ocean.
Siderophores enhance Fe-mineral dissolution and bioavailability
Next, we examined the influence of ferrioxamines on dust-Fe solubility and bioavailability, using radiolabeled amorphous Fe-oxyhydroxide – 55ferrihydrite - as a proxy for dust. In a series of dissolution and uptake experiments, we followed the iron path from the solid to the dissolved phase and into Trichodesmium and its associated bacteria, implementing a method we recently optimized for this purpose20. We added Ferrioxamine B and E (FOB & FOE), that were detected in natural blooms (Fig. 2) to the experiments and tested their effect on 55ferrihydrite dissolution rates and uptake by the consortium. Furthermore, the added FOE was extracted from one of the bacteria we isolated from natural Trichodesmium colonies from the Gulf of Aqaba. Baseline values (controls) for non-siderophore assisted dissolution and uptake rates were obtained in the presence of heat-inactivated ferrioxamines.
In the absence of Trichodesmium, the ferrioxamines enhanced 55ferrihydrite dissolution rates by ~4–6-fold compared to controls with heat-inactivated siderophores (Fig. 4a–c, black diamonds), demonstrating that these compounds can dissolve Fe-minerals present in dust. Uptake experiments with natural colonies and cultures showed that the siderophore enhanced dissolution directly benefited the Trichodesmium consortium, with up to 10-fold higher uptake rates compared to the controls (Fig. 4). Remarkably, Fe uptake rates were of the same order of magnitude as the observed dissolution rates, which imply that all the iron that was dissolved from ferrihydrite was assimilated (Fig. 4a–c). In the cultures, both ferrioxamines had a strong and positive effect on Fe uptake (Fig. 4c), while in natural colonies FOE had a much stronger positive effect on Fe-uptake than FOB (Fig. 4a, b). These differences may reflect the larger exposure of natural colonies from the Gulf of Aqaba to FOE compared to FOB (Fig. 3b–d), and possibly hint at specificity in the uptake systems. This observation is supported by studies showing that Trichodesmium indeed has proteins capable of siderophore transport33. Interestingly, siderophore additions enhanced Fe-uptake in natural Trichodesmium to a greater extent than for associated bacteria (Fig. 4d–f). The lack of benefit for the bacteria from the added siderophores may indicate that these siderophore producers are sufficiently supplied with Fe even without the additions. Yet their siderophore production is also favorable for themselves, as enhanced Trichodesmium growth favors associated bacteria via production of exudates2,3.
The confined colony core is favorable for mineral-Fe uptake
Particles concentrated in the colony core provide a localized source of mineral-Fe that can be dissolved by siderophores. Hence, Trichodesmium and bacteria cells in the colony core may benefit from proximity to dissolved Fe and can internalize more Fe than cells in the colony periphery. We tested the spatial distribution of Fe internalized by several natural Trichodesmium colonies that were incubated for 24 h with 100 nM 55ferrihydrite, using radio imaging. Indeed, internalized Fe was detected mostly in the colony core and less in the periphery (Fig. 5). This finding of mineral-Fe utilization in the colony core further demonstrated how this consortium overcomes various physical and chemical constrains related to mineral-Fe availability. Trichodesmium combines physiological and behavioral traits enabling it to encounter, capture, and center dust within a microenvironment, where, assisted by its colony microbes, it dissolves mineral-Fe and effectively acquires it before it is lost by diffusion.
Trichodesmium colonies form a cohabitation that actively reacts to dust by capturing it and extracting its nutrients. The colony microenvironment is an ideal physical setting for siderophore-mediated dissolution, which is highly effective under low turbulence, high bacterial density, and short-range organism-mineral interactions. Trichodesmium’s ability to trap dust and confine it in the colony center, provides an optimal environment for dust dissolution by siderophores, allowing buildup of high siderophore concentrations with minimal diffusive losses of siderophores, either free or as the Fe containing complex34. The confined colony microenvironment is also favorable for buildup of quorum sensing molecules known to play a role in coordinating siderophore production by different bacteria35,36. In addition, low carbon and nitrogen resources likely limit siderophore production by free-living bacteria, while Trichodesmium colonies provide the substrate for bacterial colonization and large fluxes of carbon and nitrogen in the form of exudates2,3. Hence, bacteria residing within Trichodesmium colonies have a distinct advantage over free-living bacteria in dissolving and utilizing Fe-minerals. In return, Trichodesmium gains a source of bioavailable dissolved Fe that would have otherwise remained as insoluble dust-bound Fe. We conclude that the collaborative effort within Trichodesmium colonies to increase bioavailability of iron from dust is mutualistic.
Our study is unique among the many genomic-based attempts to untangle the complex Trichodesmium-bacteria interactions, because it provides direct experimental evidence for the actual components of dust-Fe acquisition by the consortium. We confirmed genomic predictions of siderophore production and uptake by the consortium members26 and support the concept that Trichodesmium and its colony microbes act as a synchronized metabolic circuit sharing key resources13. Our study adds to a growing body of research indicating that interspecies interactions control cycling of nutrients, such as nitrogen, carbon, phosphorus, iron, and vitamin B1237,38. The microbial interactions within the colonies expand Trichodesmium’s metabolic diversity and contribute to their success in oligotrophic systems14,37,38.
In the open ocean, Trichodesmium is often co-limited by Fe and P and relies on dust inputs to supplement the supply of these limiting nutrients39. Wind-driven dust deposition into the oceans is predicted to intensify due to global warming driven desertification15. These future climatic scenarios of increased particulate Fe (and possibly P) inputs from dust deposition are favorable for Trichodesmium, owing to the unique mining strategies of Fe from dust elucidated in this study. As a result, in the future ocean Trichodesmium may increase in abundance and by nourishing other phytoplankton with essential nutrients can accelerate ocean primary production and biogeochemical cycling of elements.
Collection of individual Trichodesmium colonies and surface blooms
Samples were obtained from two study sites: the coastal Arabian Sea)15.448°N, 73.767°E) and the Gulf of Aqaba, at the northern end of the Red Sea (29.501°N, 34.917°E). In the Arabian Sea natural Trichodesmium bloom was collected from surface waters with a small boat in April 2014. In the Gulf of Aqaba several transient Trichodesmium blooms were observed next to the pier of Interuniversity Institute for Marine Sciences during April and May 2016 (Supplementary Fig. 2). These surface blooms were collected using acid-washed wide-mouth 5L polypropylene containers, stored in Nalgene polycarbonate bottles and were immediately closed and transferred to laboratory. During spring (March-April) of 2016, individual Trichodesmium colonies were also collected from the Gulf of Aqaba using a static 200 µm pore-size net as described earlier20. In brief, Trichodesmium colonies were handpicked from polypropylene containers, examined for integrity under stereoscope and washed three times in chelex-cleaned filtered seawater (cFSW) prior to setting up incubation experiments. All experimental manipulations were carried out according to stringent trace metal clean protocols as further explained in the Supplementary notes.
Characterization of ferrioxamines from natural Trichodesmium blooms
Analysis of ferrioxamines: siderophores were identified and quantified by high-performance liquid chromatography – electrospray ionization mass spectrometry (HPLC-ESI-MS; Ultimate 3000 and Q Exactive, Thermo Scientific) following preconcentration via solid-phase extraction24,28. Between 300 and 500 mL of incubated sample was preconcentrated over 200 mg ENV+ solid-phase extraction cartridges (Biotage) at ambient pH. Prior to analysis, columns were defrosted, washed with 10 mM ammonium carbonate (pH 8.3), and eluted with 5 mL of acetonitrile: propan-2-ol: water: formic acid (80:15:5:0.1 v:v:v:v). A 1-mL aliquot was evaporated in a centrifugal evaporator (Thermo) to a volume of ~100 µL and then diluted with 1 mL 0.1% formic acid. Weights were recorded to allow for accurate calculation of the preconcentration factor. The sample was split into four aliquots. Three aliquots were used for determination of ferrioxamine concentrations by standard addition. Standard addition was performed after addition of ferrioxamine B (Sigma), ferrioxamine G, and ferrioxamine E (EMC micro-collections, Germany). The concentrations of ferrioxamine G and E were standardized against ferrioxamine B by HPLC coupled to ICP-MS (Element XR, Thermo) after Boiteau et al.40. Expressed concentrations do not account for losses during preconcentration and are therefore a minimal estimate. Procedural blanks comprised of evaporated extract of ENV+ cartridges were determined to be 0.27 ± 0.11 nmol L−1 FOB, undetectable for FOG and 0.3 ± 0.07 for FOE (n = 6). One aliquot of the extract was spiked with Ga to generate Ga-siderophore complexes for isotopic mining41.
Incubation set-up and filtration: bloom samples were washed and transferred to 500 mL cFSW for incubations (Supplementary Fig. 2). Uniform distribution of colonies was achieved by preparing a stock inoculum of hand-picked colonies that was added to 1 L Nalgene bottles. Siderophores production was then examined in the incubations over 1–2 days (25 °C, 12:12 h photoperiod with ~80 µmol m−2 sec−1) in the absence and presence of dust collected at the Gulf of Aqaba (see Supplementary notes on dust collection and characterization). The dust was sterilized by UV irradiation in open petri-dishes for 30 mins to de-activate potential siderophore-producing microbes. The UV-sterilized dust was further washed in cFSW to remove potential contaminants and siderophores. The same dust was used in all incubations at a concentration of 2 mg L−1. Siderophores were retained on a methanol pre-activated ENV+ cartridges at the beginning and end of the incubation. Samples were pre-filtered through a 0.22-µm Acrodisc filter and loaded on ENV+ cartridges at a slow flow rate (~3 mL min−1) using Dynamax peristaltic pump. In situ siderophores from natural non-washed Trichodesmium blooms were also retained on ENV+ cartridges in a similar manner. All cartridges were stored at −20 °C prior to extraction and shipped to Germany for further processing. Biomass of Trichodesmium and associated bacteria were monitored in all samples - Chlorophyll a and microscopic counts of Trichodesmium, and DAPI counts for bacteria, as detailed further in Supplementary notes.
Epibiont isolation, siderophore screening, and extraction
Individual Trichodesmium colonies were repeatedly collected from Gulf of Aqaba during winter of 2014 to isolate associated bacteria. Colonies were washed thrice with microwave sterilized filtered seawater, homogenized by vortex, plated on Zobell’s 2216E solid medium, and incubated for 72 h at 25 °C. From these plates, 23 associated bacteria colonies were isolated, purified, and screened for siderophore production, using Chrome Azurol-S assay42 (Supplementary Fig. 1). Siderophores were induced by growing purified isolates in Fe-limited cFSW media amended with chelex-cleaned 0.05% tryptone, phosphate (100 µM), ammonium chloride (5 µM), and MgSO4.7H20 (50 µM), at 25 °C, shaking 150 rpm for 72 h. The cells were spun down at 10,000 rpm and 100 µL of cell-free supernatant was allowed to equilibrate with Chrome Azurol S dye in a 96-well microtiter plate (Bio-Tek) for 30 mins and absorbance measured at 630 nm. Synthetic siderophore Desferrioxamine B (1–100 µM) and blank media were used as positive and negative controls, respectively to confirm siderophore producers. The percent reduction in absorbance of Chrome Azurol S dye (630 nm) with respect to blank media was expressed as percent siderophore units.
A potent siderophore-producing epibiont strain E-23 was further grown in 1L cFSW low-Fe media and its secreted siderophores were extracted using Sep-Pak C18 columns.
In brief, stationary phase E-23 cells were spun down and the cell-free supernatant was slowly pumped through a series of three C18 Sep-Pak columns activated with methanol. The solution was circulated through the Sep-Pak three times to increase extraction yield. The columns were then eluted with three aliquots of 5 mL methanol, dried overnight in a laminar flowhood over ice, and reconstituted in 1 ml of 18.2 MΩ.cm DDW. The presence of siderophores in the C18 extract was confirmed using the Chrome Azurol-S assay. HPLC-ESI-MS analysis of this extract detected a single Fe-binding ligand, identified as ferrioxamine E (Supplementary Fig. 3). Addition of 69Ga showed that ferrioxamine E was the dominant siderophore, although trace amounts of ferrioxamine G were also observed. This C18 extract was not purified thereafter by preparatory chromatography, its concentration was determined using the Chrome Azurol-S assay and the extract is referred to as FOE in this publication.
Probing the effect of siderophores on mineral Fe-uptake and dissolution rates
We used a recently optimized radiotracer assay with 55ferrihydrite mineral to test the effect of siderophores on 55ferrihydrite dissolution and uptake by natural and cultured Trichodesmium and bacteria, which is fully detailed in Basu and Shaked20.
Mineral iron dissolution assay: radiolabeled iron oxyhydroxide (amorphous ferrihydrite) was synthesized by titrating acidic 55Fe solution (55FeCl3, specific activity 10.18 mCi mg−1, Perkin Elmer) with 0.1 N NaOH to pH 8.1. The amorphous mineral that formed was stabilized by heating (60 °C, 2 h) and subsequent aging for 3 weeks Dissolution rates were measured in cFSW over 24 h at 25 °C in the absence of cells using 100 nM 55ferrihydrite, by examining the fraction smaller than 0.22 µm. Sub-samples were filtered through 0.22 µm polycarbonate filter at the beginning and end of the incubation (or in additional intermediate time points). Aliquots were placed in Quick-Safe scintillation cocktail for β-counting in Tri-carb 1600 CA (Packard) liquid scintillation counter.
Mineral iron uptake assay: iron internalization rates were measured by incubating either natural Trichodesmium (30–40 colonies per treatment) or Fe-limited cultured Trichodesmium erythraeum ISM101 (1.7 ± 0.22 × 104 trichomes mL−1) with radiolabelled 100 nM 55ferrihydrite for 24 h (25 °C, 12:12 h photoperiod with ~80 µmol m−2 s−1)20. At the end of the incubation Trichodesmium was washed in Ti-EDTA-citrate reagent (10–20 mins) to remove all extracellular 55ferrihydrite. This wash not only dissolves the 55ferrihydrite adsorbed on the cells, but it also enables effective separation between bacteria and Trichodesmium due to loosening of the colony structure. We then collected Trichodesmium trichomes and bacteria cells separately and on 8 and 0.22 μm polycarbonates membranes, respectively. Internalized Fe by the cells on the membranes were detected using scintillation counting as above. Prior to the experiments, we examined the separation efficiency of bacteria from Trichodesmium and estimated that 2–5% of the bacteria that were initially present in the colonies remained with the washed Trichodesmium. These bacteria may account at most for 5% of the 55Fe uptake signal of Trichodesmium and hence are within the experimental error (Supplementary Table 3 and Supplementary Notes). In order to probe for the effect of siderophores on dissolution and uptake of mineral Fe, we added 1 µM of ferrioxamine B and E to the assays. Ferrioxamines B was commercially available (Sigma D9533), while ferrioxamines E was extracted from a bacterial strain E-23 isolated from natural colonies (see above). Heat-inactivated ferrioxamines were used as controls for the assays. The heat-inactivated ferrioxamines (dry heat at 120 °C for 2 h43) showed no Chrome Azurol-S activity.
Radio-imaging of 2D mineral Fe uptake
Individual Trichodesmium colonies were incubated with 100 nM 55ferrihydrite in a 96-well microtiter plate for 24 h. The colonies for radio imaging were carefully picked and soaked in Ti-EDTA-Citrate solution for 10 min to remove extracellular ferrihydrite. The colonies were fixed with 2% buffered glutaraldehyde (v/v), washed by five repeated transfers in fresh FSW using droppers and placed on glass-slides. Here, by minimizing physical manipulation we managed to retain some intact colonies for the imaging. Killed individual colonies were treated as such to confirm absence of intracellular 55Fe. The slides were covered by a scintillation foil and the internalized 55Fe was radio-imaged using a Beta-Imager (Biospace Lab, Nesles la Vallée, FRANCE). Contrary to autoradiography by photographic emulsions, this method is quantitative, and imaging was terminated once 2 million counts were achieved (~3 weeks). The images were analyzed using M3Vision to determine the uptake in CPM mm−2. Radio-images of internalized Fe were superimposed on the colony photographs and presented as in Fig. 5 (main-text). The imaged 55Fe map represents uptake by Trichodesmium cells and not uptake by the small fraction of bacteria that escaped the separation procedure, since the amount of 55Fe within these bacteria is negligible (see above). Dead controls (killed with 2% glutaraldehyde for 2 h) were also imaged and revealed no measurable counts above background.
Statistics and reproducibility
Given the low biomass of naturally occurring Trichodesmium population, we were unable to replicate all our measurements, let alone exceed two replicates (duplicates). However, the reproducibility of our results was attained by repeating the siderophore measurements and the uptake experiment over multiple days during two seasons, and in two remote sites. When experimenting with Fe-limited cultured Trichodesmium erythraeum strain IMS101, five biological replicates were conducted to ensure statistical significance.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
All data supporting the findings of this study are available in the Supplementary Information file.
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We thank Eric Achterberg (GEOMAR, Germany) for critical inputs during writing the manuscript and Murielle Dray for valuable technical assistance during the study. This study was supported by German-Israeli Foundation for Scientific Research and Development (www.GIF.org.il) and in part by Israel Science Foundation grant 458/15 (www.isf.org.il) awarded to Y.S.; S.B. acknowledges PBC Postdoctoral fellowship for Indian researchers (2014-17) to carry out this work.
The authors declare no competing interests.
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Basu, S., Gledhill, M., de Beer, D. et al. Colonies of marine cyanobacteria Trichodesmium interact with associated bacteria to acquire iron from dust. Commun Biol 2, 284 (2019). https://doi.org/10.1038/s42003-019-0534-z
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