Fig. 1 | Communications Biology

Fig. 1

From: Forecasting cell fate during antibiotic exposure using stochastic gene expression

Fig. 1

Differences in single-cell carbenicillin susceptibility. a Snapshots of cells demonstrate variable lethality of carbenicillin. PgadX-cfp fluorescence (cyan); propidium iodide is a cell death marker (red). b Cellular populations die progressively after carbenicillin exposure. Line represents mean killing curve as a function of time. Shaded region represents standard deviation across five replicate microscopy positions containing ~100 cells each. Cartoon schematic demonstrates how lethality is variable among individuals within the population, but depends on initial PgadX-cfp fluorescence. c Cells die at different times as a function of their initial PgadX-cfp fluorescence. The x axis shows the cumulative percentage of dead cells at each time point. Initial fluorescence at t = 0 is split in deciles with equal numbers of cells in each of the ten bins along the y-axis (Supplementary Fig. 2). d Population-level carbenicillin killing curves for cultures containing a plasmid expressing gadX or cfp. Carbenicillin-killing curves for wild type and ΔgadX cultures. For both data sets, n = 3 biological replicates and error bars show standard error of the mean

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