Fig. 4 | Communications Biology

Fig. 4

From: Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device

Fig. 4

Characterization of free-floating and total miR-21 in healthy human plasma using the integrated microfluidic platform and benchmarked against conventional protocol using RT-qPCR. a The voltage shift ΔV for each analysis both before (free-floating) and after (total) SAW-induced EV lysis showing consistent results across the three donors. b Free-floating and total miR-21 expression level in healthy human plasma using both the integrated microfluidic platform and RT-qPCR protocol. For the expression level, the mean free-floating value measured by both the integrated device and RT-qPCR has been set to 1.0, and lysed exosome measurements are referenced to these values, respectively. Two different donor cohorts, each consisting of three healthy human plasma samples, were used for the microfluidic platform (donors 1–3) and RT-qPCR protocol (donors 4–6), respectively. For both panels, the mean reflects the average of all nine data points, and the error bars represent the uncertainty at 95% confidence

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