Fig. 1 | Communications Biology

Fig. 1

From: Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device

Fig. 1

Design and operation of integrated microfluidic platform for EV-miRNA analysis. a The device consists of two chips, a lysing chip and concentration/sensing chip, connected by a simple transfer tube such that sample is analyzed under continuous flow. b The principle of lysing using SAWs, where the SAWs refract into the microchannel and generate an acoustic pressure that shears the EVs open. SAWs are induced by 20 interdigitated electrodes on the surface of a 128° YX lithium niobate (LiNbO3) piezoelectric substrate with a polycarbonate microchannel attached to the surface using double-sided Kapton® tape. c The principle of IEM concentration, where a voltage is applied between two reservoirs separated by a CEM, creating an accumulation of negative space charge, including negatively charged miRNA, upstream of the CEM. d The principle of IEM sensing, where an AEM is functionalized with oligo probe complements of a target miRNA. After miRNA hybridization, the current–voltage relationship across the AEM sensor is shifted in the over-limiting region, and the voltage shift (ΔV) can be directly correlated with miRNA concentration

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