Table 1 Summary of binding data obtained from kinetic analysesa

From: Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells

Ligand on surface Analyte injected Bmax (RU) KD (nM)
TLR4 Wnt5a 226 ± 0.9 44.5 ± 2.0
Wnt3a (147 ± 30)b (166 ± 43)b
TLR4/MD2 Wnt5a 328 ± 1.8 35.5 ± 5.1
Wnt3a 170 ± 1.2 36.1 ± 3.0
TLR2 Wnt5a 274 ± 1.5 26.5 ± 4.5
Wnt3a 158 ± 0.1 41.0 ± 0.2
  1. aBinding curves [responses at steady-state (Req) vs. analyte concentration] were fit to a one-site hyperbola model to calculate Bmax (Response in resonance units (RU)) and KD (± % SE).
  2. bWnt3a showed an almost linear increase in binding to TLR4, explaining the poor fit