Fig. 3 | Communications Biology

Fig. 3

From: Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells

Fig. 3

Wnt5a and TLR4 co-localize in NIH3T3 cells. NIH3T3 cells were co-transfected with HA-Wnt5a-pcDNA3 and pUNO-hTLR4-GFP for 48 h. a Cells only transfected with pUNO-hTLR4-GFP, TLR4 was dispersed. b Cells transfected with both HA-Wnt5a-pcDNA3 and pUNO-hTLR4-GFP, TLR4 and Wnt5a co-localized, forming a cap. HA-Wnt5a was detected using HA-specific Alexa Fluor 594 antibodies; DAPI Vectashield was used for nuclear staining. The images are representative of three replicates. The scale bar represents 10 μm

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