Fig. 6 | Communications Biology

Fig. 6

From: Evidence-based guidelines for controlling pH in mammalian live-cell culture systems

Fig. 6

Summary of buffering regimes in commercially-available media formulations, and flow chart showing instructions for preparing media at a target pH. a Venn diagrams summarizing the commercial availability of Dulbecco’s modified Eagle’s medium (DMEM), minimum essential medium (MEM) or RPMI-1640 buffers (supplied by Sigma-Aldrich and Thermo Fisher Scientific), grouped by buffering regime. Area is proportional to the number of media available in each category. Media with physiological HCO3 and no additional non-volatile buffer are highlighted with a thick black border. HEPES-buffered media are indicated with a red outline. b Flow chart guiding through the steps required to adjust the pH of culture media. NVB: non-volatile buffer (e.g. HEPES). Target [HCO3] for a given medium pH can be calculated from Eq. 3. Total osmolality can be approximated as 2 × [NaCl] + 2 × [NaHCO3] + 2 × [KCl] + [Glucose]. See Supplementary Data 1 for further details of these steps

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