Fig. 2 | Communications Biology

Fig. 2

From: Carbon replicas reveal double stranded structure of tight junctions in phase-contrast electron microscopy

Fig. 2

The double-strand structure of tight junction fibrils and distinct structural organization. a Claudin-11 fibrils visualized by unidirectional platinum shadowing freeze-fracture of transfected HEK293T cells. b Higher magnification of a single claudin fibril demonstrating platinum decoration. c The power spectrum and calculated radial intensity profile (d) show characteristic polycrystalline rings consistent with the spatial frequencies associated with crystalline platinum. Unidirectional carbon freeze-fracture of HEK293T cells expressing claudin-11 and connexin 26 imaged using phase plate (e) or single sideband imaging (f). Carbon replicas feature a double-lined morphology (arrowheads) that can be observed at near focus (g), with phase plate (h), and with single sideband imaging (i), while platinum replicas show a single-lined morphology (j). k Average intensity profiles through claudin-11 strands in platinum replicas (solid black) and carbon replicas imaged using a phase plate (dotted) and single sideband (solid gray). Scale bars = 100 nm for a, e, f, 10 nm for b, gj, 2 nm−1 for c

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