Fig. 8 | Communications Biology

Fig. 8

From: SLO potassium channels antagonize premature decision making in C. elegans

Fig. 8

Epilepsy-related mutations potentiate SLO-2. a slo-2(nf101) animals expressing either wild-type or the indicated mutant form of SLO-2b in AFD were cultivated at 17 °C for 5 days and then at 23 °C for 3 h and then subjected to thermotaxis assay. Fractions of animals are plotted (upper). n = 9, 17, 16, 12, 4, 4, 2, 4, 2, 4. Thermotaxis indices of strains marked with distinct alphabets differ significantly (p < 0.05) according to Tukey–Kramer test (lower). b slo-2(nf101) animals expressing either wild-type or the indicated mutant form of SLO-2b in AFD were cultivated at 17 °C for 5 days and then at 23 °C for the indicated time points. The animals were then subjected to thermotaxis assay. The means of thermotaxis indices are shown. The error bars represent the SEM. Data at 3 h are identical to those in a. **p < 0.01, ***p < 0.001 (Tukey–Kramer test, compared with animals expressing SLO-2b(+)). The fractions of animals and individual indices at each time point are shown in Supplementary Fig. 7. c Animals expressing SLO-2b(R376Q) in AFD with either a wild-type or cng-3(nj172) background were cultivated at 17 °C for 5 days, then at 23 °C for the indicated time points, and then subjected to thermotaxis assay. Thermotaxis indices at each time point are shown. The horizontal bars represent the medians. n = 2, 6, 3, 5, 3, 3 for each time point. *p < 0.05, **p < 0.01 (Welch two-sample t test between two strains at each time point). The fractions of animals are shown in Supplementary Fig. 7. d A model for regulation of the timing of preference transition in thermotaxis is shown. SLO K+ channels and CNG-3 generate latency for preference transition in thermotaxis after an upshift in cultivation temperature by acting in AFD to slow down the AFD adaptation to new temperature. See also Supplementary Figs. 2, 3, 7, and 8

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