Fig. 7 | Communications Biology

Fig. 7

From: SLO potassium channels antagonize premature decision making in C. elegans

Fig. 7

Endogenous SLO K+ channels slow down AFD adaptation. ae Wild-type (a), slo-2(nj131gf) (b), slo-1(eg142) (c), slo-2(nf101) (d), and slo-1(eg142); slo-2(nf101) (e) animals that express GCaMP3 and tagRFP in AFD were cultivated at 17 °C for 5 days and subjected to Ca2+ imaging analysis with a temperature stimulus involving 20 s (0.05 Hz) oscillations around 17 °C followed by a temperature upshift to 23 °C and oscillations around 23 °C. The intensity of green fluorescence was divided by that of red fluorescence, and the ratio was normalized to a range between 0 and 1 and plotted against time. Gray and red lines indicate traces from individual animals and the average, respectively. Data were collected from distinct animals (n = 6). fj The Fourier transform was computed with the Hanning window on the temperature program and ratio of fluorescence intensity between 401 and 1390 s for each animal in ae. Averaged power spectra are plotted against frequency. ko Time evolutions of averaged spectrograms are plotted in a color map against the centric time of each segment. Trends detected by Butterworth filter were removed from the ratio of fluorescence intensity of each animal in ae. The resulting signals were divided into segments of 128 s, and the Fourier transform was separately computed for each segment

Back to article page