Fig. 6 | Communications Biology

Fig. 6

From: SLO potassium channels antagonize premature decision making in C. elegans

Fig. 6

SLO K+ channels slow down AFD adaptation. af Wild-type (a, n = 8, 13, 7), slo-2(nj131gf) (b, n = 8, 31, 10), cng-3(jh113) (c, n = 8, 8, 8), and cng-3(jh113); slo-2(nj131gf) (d, n = 7, 10, 9) animals; animals expressing SLO-2b(H159Y) in AFD (e, n = 6, 7, 6); and cng-3(jh113); slo-2(nj131gf) animals expressing CNG-3 in AFD (f, n = 6, 6, 6) that expressed R-CaMP2 in AFD were cultivated at 17 °C for 5 days, at 17 °C for 5 days and then at 23 °C for 3 h or at 23 °C for 3 days. Animals were then subjected to Ca2+ imaging analysis. The animals were initially kept at 14 °C for 40 s and then subjected to a linear temperature rise to 24 °C over 200 s followed by 90 s of incubation at 24 °C. Temperature stimuli and changes in fluorescence intensity are indicated with blue and red lines, respectively. Data were collected from distinct animals. Pale red shadow represents the SEM. g The onset temperatures at which the AFD response reached half of the maximum during the above experiment (af) are plotted. The onset temperatures of strains marked with distinct alphabets differ significantly (p < 0.05) according to Tukey–Kramer test under the same cultivation conditions. h Wild-type and slo-2(nj131gf) animals that express R-CaMP2 in AFD were cultivated at 17 °C for 5 days and then at 23 °C for the time indicated. Animals were then subjected to Ca2+ imaging with the same temperature increase stimulus as in af. Onset temperature is plotted, and medians are indicated with horizontal bars. Data at 0 and 3 h are identical to those in a, b. n = 3, 3, 4, 6, 4 for wild-type and n = 10, 8, 9, 8, 7 for slo-2(nj131gf) animals at each time point (6, 9, 12, 18, and 24 h). ***p < 0.001 (Welch two-sample t test between wild-type and slo-2(nj131gf) animals)

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