Fig. 5 | Communications Biology

Fig. 5

From: SLO potassium channels antagonize premature decision making in C. elegans

Fig. 5

cng-3 loss-of-function suppresses decelerated preference transition in slo-2(nj131gf) mutants. af Wild-type (a, n = 3, 3, 5, 3, 3, 6, 8), slo-2(nj131gf) (b, n = 3, 9, 5, 5, 3, 3, 8), cng-3(nj172) (c, n = 3, 3, 3, 3, 3, 5, 4), cng-3(nj172); slo-2(nj131gf) (d, n = 3, 9, 5, 5, 3, 3, 4), cng-3(jh113) (e, n = 5, 3, 3, 3, 3, 5, 6), and cng-3(jh113); slo-2(nj131gf) (f, n = 3, 3, 3, 3, 3, 5, 6) animals were cultivated at 17 °C for 5 days and then at 23 °C for the time indicated or constantly at 23 °C for 3 days. Animals were then subjected to thermotaxis assay. The fractions of animals are plotted. g The means of the thermotaxis indices at each time point (af) are plotted against time after cultivation temperature was changed to 23 °C. The error bars represent the SEM. *p < 0.05, **p < 0.01, ***p < 0.001 between wild-type and slo-2(nj131gf) animals, and p < 0.05 between slo-2(nj131gf) and cng-3(nj172); slo-2(nj131gf) animals (Tukey–Kramer test). h The thermotaxis indices for animals cultivated constantly at 23 °C in af are shown. The indices of strains marked with distinct alphabets differ significantly (p < 0.001) according to Tukey–Kramer test. i Genomic PCR fragments covering the cng-3 locus were injected into cng-3(nj172); slo-2(nj131gf) animals. Animals were cultivated first at 17 °C, then at 23 °C for the time indicated, and then subjected to thermotaxis assay. n = 5, 8, 5. ***p < 0.001 (Tukey–Kramer test). j cng-3(nj172); slo-2(nj131gf) animals expressing cng-3 under control of the indicated promoters were cultivated at 17 °C for 5 days, then at 23 °C for 12 h, and then subjected to thermotaxis assay. n = 8, 8, 6, 6, 5. ***p < 0.001 (Dunnett test against cng-3(nj172); slo-2(nj131gf) animals). See also Supplementary Figs. 5 and 6

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