Fig. 5 | Communications Biology

Fig. 5

From: Proliferative heterogeneity of murine epithelial cells in the adult mammary gland

Fig. 5

Two distinct diestrus stages occur in the mammary gland. a Schematic of workflow: Daily sequential injection of mammary glands with CFSE starting at the beginning of diestrus, to analyse in vivo cell division by CFSE dilution. b Histogram overlays show flow cytometry analysis of in vivo CFSE dilution, representing proliferation of basal, HR− and HR+ luminal cells within one estrous cycle. The green histograms show the CFSE intensity in mammary epithelial cells 48 h after injection 1, representing time-point zero (t = 0). The black dotted lines correspond to the median MFI of t = 0. The upper panels depict group 1, a high-proliferative estrous cycle, and the lower panels show group 2, a low proliferative estrous cycle. Histogram distributions were offset vertically for easy visualization. c Line diagrams represent the percentage of CFSE high events after subsequent injections calculated for each group (mean ± SEM, group 1 n = 3–5, group 2 n = 3). Student’s t-test analysis was performed. * represents statistical significance of P < 0.05

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