Fig. 4 | Communications Biology

Fig. 4

From: Proliferative heterogeneity of murine epithelial cells in the adult mammary gland

Fig. 4

Diestrus heterogeneity in the mammary gland. a Flow cytometry analysis calculating the absolute number of BrdU+ cells from mammary epithelial subsets at estrus (n = 7) or diestrus (n = 6) showing a threefold (HR+) to ninefold (HR−) higher proliferation index at diestrus. b Representative immunofluorescent images of Ki-67+mCherry+ cells from an estrus, low-proliferative diestrus and a high-proliferative diestrus mammary gland. Scale bar = 25 µm, and quantification of Ki-67+ and mCherry+ cells in mammary glands from mice in estrus (n = 3) and diestrus (n = 9). c Representative FACS plot from Fucci2 mammary glands from estrus, non-proliferative diestrus and proliferative diestrus mice showing the proportion of cells in different phases of the cell cycle in basal, HR− and HR+ luminal populations. Bar graphs indicate the relative proportion of each cell cycle population within the (d) basal, (e) HR−, and f HR+ compartments from mice in estrus (e; n = 5), diestrus (d; n = 5) and high-proliferative diestrus (D-Ex; n = 5) stages (mean ± SEM). Two-way ANOVA analysis with Bonferroni correction used to determine significance between cell cycle phases in the basal, HR− and HR+ populations. *** represents statistical significance of P < 0.001 of D-Ex compared to estrus and diestrus stages. g Immunofluorescent images of PR+Ki-67+ cells from proliferating ductal and lobule structures. Arrows indicate PR+Ki-67+ cells. Scale bar = 25 µm and quantification of PR/Ki-67 expression (n = 7). h Immunofluorescent images of PR+ cells during S-phase in lobule and ductal structures of normal proliferating mammary glands. Arrows indicate PR+BrdU+ cells. Scale bar = 25 µm and quantification of PR/BrdU expression (n = 5)

Back to article page